Background:The objective of this study was the genetic characterization of remaining populations of the species Paratecoma peroba occurring in fragments of the Atlantic Forest, by estimating parameters of diversity and genetic structure. The study was carried out in two forest fragments, the Atlantic Forest Environmental Education Pole (area 1) and the Pacotuba National Forest (area 2), where 93 adult trees were identified.Results: Ten Inter Simple Sequence Repeats (ISSR) primers were used in genotyping, obtaining 112 amplified bands with 87.5% polymorphism. The genetic diversity estimated for the populations from the Nei (H*) and Shannon (I*) indices was higher for area 1. For the joint data, moderate genetic diversity was observed, referring to the average values of 0.26 and 0.40, obtained for the H* and I* indices, respectively. Molecular variance analysis resulted in moderate differentiation between populations (ΦST = 0.143), while gene flow analysis (N m = 6.69) revealed the occurrence of similar alleles between them. However, the predominance of a single genetic group in area 2, revealed from Bayesian approach, indicates that its genetic structure, possibly generated by the current scenario of fragmentation of the Atlantic Forest and the distancing of populations, affecting the contemporary gene flow. Conclusion:Despite the moderate genetic diversity of the species for the area 2 population, actions toward inclusion of seedlings obtained from seeds from neighboring fragments, including area 1, and the increase in the connectivity of forest fragments through ecological corridors, could assist in augmenting its genetic variation.
A história do Brasil é inteiramente ligada a espécies nativas que moveram a economia do país por décadas, entre essas espécies está o jacarandá-da-bahia (Dalbergia nigra Vell. Fr. All. Ex Benth), cuja madeira possui alto valor comercial. Sua exploração aliada à falta de manejo levou essa espécie a figurar na lista das espécies ameaçadas de extinção da flora brasileira. Diante do exposto, o objetivo do presente trabalho foi avaliar as condições mais favoráveis à germinação e estabelecimento in vitro de jacarandá-da-bahia, a fim de possibilitar posteriormente a micropropagação e produção de plântulas viáveis. As sementes foram submetidas a dois tratamentos para desinfestação, uma com hipoclorito de sódio outro com peróxido de hidrogênio. Foi realizado um teste de germinação com três meios de cultivo (MS, WPM e B5) sendo o delineamento inteiramente casualizado. O tratamento com peróxido de hidrogênio apresentou maior porcentagem de germinação (72%). Os resultados de Parte Aérea (PA), Comprimento da Raiz (CR), e Massa Fresca (MF) não diferiram estatisticamente. Quanto ao número de folhas, o Meio WPM apresentou maior número de folhas, não distinguindo estatisticamente do Meio B5 (4.31 e 4.15).
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