Background Primary caregivers are those who are concerned with the client care in the hospital and home. Most of the caregivers are not able to provide stoma care with quality to their children. Hence, caregivers need to be provided with ongoing education and support through partnership commencing from preoperative teaching to discharge from the hospital and home care .The purpose of this study was to assess the effect of partnership based program with primary caregivers of children on stoma care. The design of this study was quasi-experimental design. The study was conducted in the Pediatric surgical department and outpatient clinics at both Menoufia University Hospital and Benha Specialized Pediatric Hospital. The study sample was composed of 60 children and their caregivers were selected from previously mentioned settings (30 studies and 30 control group). Five instruments were used in the study. They were Social Characteristics Structured Questionnaire, Structured Interview Questionnaire, Stoma Care Checklist, Stoma Assessment Sheet and Quality of Partnership Likert Scale. The results of this study showed that caregivers had higher mean score of knowledge posttest in the study group compared to control group (61.30 ±5.2 Vs. 14.63 ±5.7 respectively) In addition, children had better stoma clinical condition in the study group compared to control group as well as caregivers higher levels of partnership quality on post and follow-up intervention compared to pre intervention (96.7%, 100.0 % vs. 6.7%, respectively).. Conclusion: implementation of partnership based program increased caregivers' knowledge and practices regarding care of children with stoma. Therefore, it was recommended that Partnership based program for stoma care should be urgently planned and implemented to improve caregivers' knowledge and practice as well as improve children health status.