The aim of this study was to determine the relationship between general health and job burnout of male and female high school teachers in Zabol. The research followed a cross-correlational method. The statistical population included all male and female high school teachers in the academic year 2014 in Zabol. In this study, considering participants' gender, stratified random sampling method was used. Data collecting was performed through applying the Maslach's Burnout Inventory and General Health Questionnaire (GHQ-28). The content validity was checked and alpha coefficients for the variables of general health and job burnout were 0.92 and 0.81, respectively. In this research study, Pearson correlation coefficient, regression analysis, and independent t-test were conducted to analyze the hypotheses using SPSS v.21. Results revealed that general health and its dimensions were significantly and positively correlated with job burnout of male and female high school teachers in Zabol. Additionally, emotional exhaustion and depersonalization were significantly and negatively related to general health. Regarding the priority of the predictor variables of teachers' general health, in the first step, emotional exhaustion, in the second step, depersonalization and in the third step, lack of personal accomplishment entered into the prediction model, these three variables together predicted 21% of variations in general health. Moreover, general health and job burnout were not significantly correlated in terms of gender.