CBP-93872 suppresses maintenance of DNA double-stranded break-induced G2 checkpoint, by inhibiting the pathway between ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) activation. To examine the potential use of CBP-93872 for clinical applications, we analyzed the synergistic effects of platinum-containing drugs, oxaliplatin and cisplatin, pyrimidine antimetabolites, gemcitabine and 5-fluorouracil (5-FU), in combination with CBP-93872, on cell lethality in colorectal and pancreatic cancer cell lines. Treatment with CBP-93872 significantly increased cancer cell sensitivities to various chemotherapeutic agents tested through suppression of checkpoint activation. Our results thus reveal that combination treatment of CBP-93872 with known chemotherapeutic agents inhibits phosphorylation of ATR and Chk1, and induces cell death.

No abstract

Aim Robotic surgery using the da Vinci system has markedly increased worldwide. However, robotic inguinal hernia repair remains unpopular outside the United States. We introduced and evaluated a robotic transabdominal preperitoneal repair (R‐TAPP) technique for inguinal hernia in our hospital. Methods First, we designed a task protocol according to the surgical results of 388 laparoscopic TAPP (L‐TAPP) procedures performed during the 4 years prior to introducing R‐TAPP. Our task protocol included several time limitations during a step‐wise procedure: creating the peritoneal flap (<60 minutes), mesh placement with fixation (<30 minutes), and peritoneal suture closure (<30 minutes) under experienced supervision. We investigated the preliminary clinical results of R‐TAPP performed by a single operator between December 2018 and January 2020. Results We identified 27 lesions in 20 patients (unilateral in 13 and bilateral in seven). According to the Japan Hernia Society Classification, our cohort included eight type I, five type II, and seven bilateral hernias (nine type I, four type II, and one type IV). The median operation time was 124 minutes (range, 81‐164 minutes), and the median console operation time was 85 minutes (range, 50‐132). The median time required for the peritoneal incision was 30 minutes (range, 18‐54 minutes), that for mesh placement (including tucking) was 13 minutes (range, 7‐27 minutes), and that for peritoneal suturing was 9 minutes (range, 3‐20 minutes). Conclusion Our preliminary results suggest that our task protocol for R‐TAPP is feasible. However, refinement of our task protocol is essential for standardization.

No abstract