New Russian F1 "Rubinovaya" cultivar of Daucus carota was found to be pink-colored due to biosynthesis of lycopene and β-carotene in relatively high overall level (up to 0.200 mg per 1 g of fresh weight, FW). The typical carotenoid composition of carrot roots with pink color were determined by proposed in the paper reversed-phase HPLC method. These are all-trans-lycopene (28-47 %); mixture of cis-lycopene isomers (7-10 %), all-trans-β-carotene (30-55%) and mixture of cis-β-carotene isomers (7-10 %). From chromatographic point of view carotenoids are retained in the proposed conditions by distribution mechanism due to a long chain of conjugated C=C-bonds makes this part of carotenoids planar with ability penetrate space between grafted C18 chains.