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SUMMARYThe title compounds were synthesized for metabolic studies in plants and animals, with 14C labelling being made at position 4 of the epibrassinolides 4 and 5 in an overall radiochemical yield of 3.22% and 4.46%, respectively, based on barium [14C]carbonate.

A sensitive high performance liquid chromatography (HPLC) method with post-column chemiluminescence detection was developed for the simultaneous determination of oxycodone (OXC) and its metabolite, noroxycodone (NOC), in human plasma. Sample preparation for human plasma was performed by means of solid extraction with Sep-Pak Vac C18 (100 mg/mL). Separation was performed by reversed-phase HPLC

A one-pot synthesis of S-2474 was developed to overcome the problems of a large number of steps, low stereoselectivity, low yield, a large amount of waste, and severe reaction conditions. Aldol-type condensation of 3,5-di-tert-butyl-4-hydroxybenzaldehyde and N-ethyl-γ-sultam was carried out with LDA and then quenched with water. Dehydration proceeded under basic conditions, providing S-2474 directly as a single isomer on the benzylidene double bond. The reaction mechanism appears to involve a quinone methide intermediate. Environmental assessment of the development of this compound is also discussed in this paper.