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The baculovirus-insect cell system is effective in the production of large quantities of biologically active recombinant proteins. The secretory production of an antibody Fab fragment in lepidopteran insect cells infected with a recombinant baculovirus that contains both the heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of the Fab fragment is described in this chapter. The recombinant baculovirus can be generated rapidly and efficiently through site-specific transposon-mediated insertion of foreign genes into a baculovirus genome called a bacmid in Escherichia coli. Factors influencing the Fab fragment production are discussed.

The heavy rain disaster in the Kinugawa River basin that occurred along with the passage of the Typhoon 18 caused the embankment destruction in the middle reach of the river on September 10, 2015. Due to the overflow, the houses in the vicinity of the embankment collapsed, causing a flood inundation spreading over a wide area. Because the embankment breakwater occurred during the daytime, the state of the inundating flow was recorded from various angles by media helicopters or drones. In this study, we developed a method to extract quantitative flow information from a helicopter video image in which the shooting position and angles are changed one after another, because it was taken in emergency. In the analysis, the images were orthorectified after stabilizing the images, from which surface velocity distributions were measured by image-based technique such as the large-scale particle image velocimetry (LSPIV) or the space-time image velocimetry (STIV). As a result, the time change of water entering from the broken embankment and the total inundated water volume during the disaster were estimated. In addition, two-dimensional surface velocity distributions were analysed to show the spreading of inundated flow.

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