Homoeoalleles of Ncc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon of Aegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmp1A locus, which is located on chromosome 1A of T. timopheevi, was sequenced and converted to a PCRbased sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T. timopheevi and T. turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmp1A locus would be informative for studies of the differentiation of chromosome 1A in wheat.
The authors reported on the characteristics of wetting by capillarity of the assemblies of six types of acrylic fibers (in IV of this series).In this article, comparison is made between the wetting characteristics of the web under hydrostatic pressure and that of web immersed in water.Samples and their purification method are the same as decribed in the previous report (IV).In the experiment under the hydrostatic pressure the rate of the movement of water front dow nward through the assemblies and the rate of flow of water (volume per unit time) after the water front had reached the bottom of the assemblies were observed. Porosity of the assemblies was 75%. and the hydrostatic pressures were 15, 40 and 65 g. wt/cm2. The time required for passage of water through the assemblies of ordinary acrylics was very short and the large quantities of water flowed down per unit time from the bottom of the assemblies. On the contrary, in the case of modacrylic fiber both quantities were exceedingly small under low hydrostatic pressure.In the experiment of steeping method the relationship between the apparent density of sample (Day) and the elapsed time (TS) were obtained by means of apparatus described in the previous paper (III). The dependency of the retained air index (V5(VV), of the assemblies on the elapsed time could also be obtained from these data. The assemblies of ordinary acrylics were penetrated by water rapidly and almost perfectly.But the volume of distilled water penetrated into the ass embly of modacrylic fiber was exceedingly small. It was pointed out that the wettability of ass embly of modacrylic fiber is smaller than that of polyester (
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