Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.

A phreatic eruption of Mt. Ontake, Japan, started abruptly on September 27, 2014, and caused the worst volcanic calamity in recent 70 years in Japan. We conducted volcanic plume surveys using an electric multirotor unmanned aerial vehicle to elucidate the conditions of Mt. Ontake's plume, which is flowing over 3000 m altitude. A plume gas composition, sulfur dioxide flux and thermal image measurements and a particle sampling were carried out using the unmanned aerial vehicle for three field campaigns on November 20 and 21, 2014, and June 2, 2015. Together with the results of manned helicopter and aircraft observations, we revealed that the plume of Mt. Ontake was not directly emitted from the magma but was influenced by hydrothermal system, and observed SO 2 /H 2 S molar ratios were decreasing after the eruption. High SO 2 flux of >2000 t/d observed at least until 20 h after the onset of the eruption implies significant input of magmatic gas and the flux quickly decreased to about 130 t/d in 2 months. In contrast, H 2 S fluxes retrieved using SO 2 /H 2 S ratio and SO 2 flux showed significantly high level of 700-800 t/d, which continued at least between 2 weeks and 2 months after the eruption. This is a peculiar feature of the 2014 Mt. Ontake eruption. Considering the trends of the flux changes of SO 2 and H 2 S, we presume that majority of SO 2 and H 2 S are supplied, respectively, from high-temperature magmatic fluid of a deep origin and from hydrothermal system. From the point of view of SO 2 /H 2 S ratios and fumarolic temperatures, the plume degassing trend after the 2014 eruption is following the similar course as that after the 1979 eruptions, and we speculate the 2014 eruptive activity will cease slowly similar to the 1979 eruption.

Broadband magnetotelluric (MT) measurements were conducted in 2010 and 2011 in the vicinity of Shinmoe-dake Volcano in the Kirishima volcano group, Japan, where sub-Plinian eruptions took place 3 times during 26-27 January 2011. By combining the new observations with previous MT data, it is found that an anomalous phase in excess of 90°is commonly observed in the northern sector of the Kirishima volcano group. Because the anomalous phase is not explained by 1-D or 2-D structure with isotropic resistivity media, 3-D inversions were performed. By applying small errors to the anomalous phase, we successfully estimated a 3-D resistivity structure that explains not only the normal data but also the anomalous phase data. The final model shows a vertical conductor that is located between a deep-seated conductive body (at a depth greater than 10 km) and a shallow conductive layer. By applying the findings of geophysical and petrological studies of the 2011 sub-Plinian eruptions, we infer that the subvertical conductor represents a zone of hydrothermal aqueous fluids at temperatures over 400°C, in which a magma pathway (interconnected melt) is partially and occasionally formed before magmatic eruptions. To the north of the deep conductor, earthquake swarms occurred from 1968 to 1969, suggesting that these earthquakes were caused by volcanic fluids.

We deduced the ζ potential of various rocks in Aso caldera from streaming potential measurements in laboratory. In contrast to the conventional observation that the ζ potential of crustal rocks is mostly negative under typical geologic conditions, almost half of the samples equilibrated with dilute KCl solution show positive values of ζ potential at pH 4.5–5.5. The samples showing positive ζ are characterized by relatively low SiO2 content and abundance of elements having high isoelectric points, and found to be localized around the summit of Mt. Takadake where the positive correlation between self‐potential (SP) and altitude is observed. We emphasize that measurements of ζ potential of volcanic rocks are very important to give more appropriate interpretations of SP data from volcanic fields.

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