A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, CbxR, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.

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This paper presents a novel approach to skim and describe 3D videos. 3D video is an imaging technology which consists in a stream of 3D models in motion captured by a synchronized set of video cameras. Each frame is composed of one or several 3D models, and therefore the acquisition of long sequences at video rate requires massive storage devices. In order to reduce the storage cost while keeping relevant information, we propose to encode 3D video sequences using a topology-based shape descriptor dictionary. This dictionary is either generated from a set of extracted patterns or learned from training input sequences with semantic annotations. It relies on an unsupervised 3D shapebased clustering of the dataset by Reeb graphs, and features a Markov network to characterize topological changes. The approach allows content-based compression and skimming with accurate recovery of sequences and can handle complex topological changes. Redundancies are detected and skipped based on a probabilistic discrimination process. Semantic description of video sequences is then automatically performed. In addition, forthcoming frame encoding is achieved using a multiresolution matching scheme and allows action recognition in 3D. Our experiments were performed on complex 3D video sequences. We demonstrate the robustness and accuracy of the 3D video skimming with dramatic low bitrate coding and high compression ratio.

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