Recreational cycling is sort of sport that requires a decent level of endurance, and cyclists’ riding is determined by several physical abilities, in which muscular strength in upper limb is equally important as the lower limbs. While riding a bicycle, the hand grip turns out to be especially crucial not just for performance and the ability to remain upright in difficult situations and uneven surfaces but also for even moderate endurance and comfort of the rider A total of 60 recreational cyclists, 25-35 years old were included in the study. Grip strength of dominant and non-dominant hand was checked with Jamar hand held dynamometer pre cycling and was checked again after the 20km ride. Normality was checked and Dominant hand data passed the normality test thus, paired t-test was used to determine significance for (p<0.05). For Non-dominant hand readings didn’t support normality to the optimal level (p>0.05) hence non-parametric Wilcoxon signed test was used to determine significance as the samples were dependent. Mann-Whitney test was used to determine significance in Dominant and Non-Dominant hand pre cycling activity as well as comparison of Dominant and Non-Dominant hand post cycling activity, as data was not normally distributed and the samples were independent. (p>0.05). Results showed that there is significant difference in dominant as well as non-dominant hand pre and post riding activity with post cycling grip strength being reduced in both Dominant and Non-Dominant hand than the pre-cycling grip strength. Also, Dominant hand grip strength was more than the non-Dominant hand in both pre and post cycling. Key words: Recreational cyclists, Grip strength, dominant and non-dominant hand, Jamar hand held dynamometer.