Tuberculosis (TB) is considered one of the most prominent diseases across the globe. This present study aims to inspect the impact of silver nanoparticles (AgNP) against Mycobacterium tuberculosis, which is the causative vector of TB. The efficacy of the AgNP was conducted based on the minimum inhibitory concentration (MIC) of the AgNPs through microplate Alamar blue assay. The preparation of the AgNPs involved chemical synthesis. The state and the size of the AgNPs were determined and confirmed by using ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD) spectroscopy, and Transmission electron microscopy (TEM). This study evaluated two strains: Mycobacterium tuberculosis H37Rv and Mycobacterium bovis. In addition, another multiple drug-resistant Mycobacterium tuberculosis strain was also considered in this study, along with the clinically obtained isolates from Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis bovis. The synthesized nanoparticles were found to be tetrahedral in shape with an average particle size of 45±3 nanometre (nm). The obtained results indicated that the proliferation of all the strains (two reference strains and one MDR strain) was resisted by the action of the synthesized AgNPs. The MIC of the MDR strain was noted within the range of 2-12 µg/ml, whereas the MIC for the Mycobacterium tuberculosis H37Rv and Mycobacterium bovis was noted in the range of 2-14 and 3-30 µg/ml, respectively. Accordingly, this study proposed a novel approach to combat tuberculosis, which is considered a global threat to humankind, indicating the present study's novelty.

Our earth is composed of five different elements named air, water, fire, earth, and space. Most of the resources which have been supporting our lives, directly and indirectly, are now polluted due to various human activities which are resulted due to various societal advancements like rapid industrialization, population, etc. to name a few. Among all these, the pollution which has taken place due to these activities in turn has received notable research cognizance. The quality of water is judged by different parameters namely, physical, chemical, and biological. This study deals with the determination of the quality of water from two different point sources (industrial sewage (referred to as point source 1), and domestic sewage (referred to as point source 2)) through the determination of water quality indicators. The various quality indicators adopted in this study dissolved oxygen (DO), pH, electrical conductivity (EC), sulphates, chlorides, phosphates, total solid (TS), total soluble solids (TSS), total dissolved solids (TDS), biochemical oxygen demand (BOD5), and chemical oxygen demand (COD). The result obtained from these experiments was thought-provoking and indicated that the quality of the water specimens used in this study was far below the standards. The possible cause might be due to the various activities detailed earlier. The authors truly believe that this present scheme of work will be helpful to a spectrum of stakeholders which will indeed be helpful for decision-making by various national and international agencies.