Background Inadequate trophoblast invasion is associated with preeclampsia (PE). Ankyrin repeat domain protein 37 (ANKRD37) has been reported to be abnormally expressed in PE placentas. However, the role of ANKRD37 in trophoblasts has not been investigated. We aimed to determine the functions of ANKRD37 in PE and to explore the molecular mechanisms. Methods Here, fluorescence in situ hybridization, immunohistochemistry, Western blotting and quantitative real‐time polymerase chain reaction were used to detect protein and mRNA expression levels. Cell counting kit‐8 assay, 5‐ethynyl‐2′‐deoxyuridine assay, flow cytometry, wound healing assay, transwell assay and RNA sequencing were performed to investigate the role of ANKRD37 and the underlying mechanism in HTR8/SVneo and JEG‐3 cells, and extravillous explant cultures were used to evaluate the migration and invasion abilities of extravillous cytotrophoblasts. Results We found that ANKRD37 expression was upregulated in PE placentas compared to normal pregnancy placentas. ANKRD37 knockdown enhanced trophoblast migration and invasion, promoted extravillous explant outgrowth, and regulated the expression of key invasion proteins, whereas ANKRD37 overexpression exerted the opposite effects. RNA sequencing indicated that nuclear factor‐kappa B (NF‐κB) was the potential downstream pathway of ANKRD37, which was confirmed by the change in p‐p65 and p‐IκBα expression in JEG‐3 and HTR8/SVneo cells. Conclusions Our findings suggest that high expression of ANKRD37 inhibits trophoblast cell migration and invasion possibly via the NF‐κB pathway, and may be related to the development of PE.

Abstract. To observe the Forsen source herbal tea of reserpine in mice model of spleen deficiency of gastrointestinal effects. Mice were injected with reserpine injection to each mice by 0.1mg / kg in 17 days with continuous subcutaneous injection molding, mice dranking water solution respectively at the same time, pills, small dose, small dose of Fukumori Genryo tea, Wang Laoji, will be the corresponding herbal tea instead of mice daily drinking water; the control group was given ordinary drinking water. In 16th, mice were measured the gastrointestinal bioelectric and sacrificed at 17th. To observe the mice's bioelectricity and organ morphology changes. the spleen deficiency of mice model, compared with the model group, the large and small dose of the Forsen source herbal tea in mice bioelectricity and tissuse morphology without large the influence of the Forsen source herbal tea. No obvious adverse effect on spleen deficiency mice induced by reserpine bioelectricity and organ morphology.