Abstract:We investigated the effect of magnetic nanoparticles of Fe 3 O 4 (Fe 3 O 4 -MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe 3 O 4 -MNPs, respectively. After intravenous administration of Fe 3 O 4 -MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P . 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P , 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P , 0.05). In peripheral blood, both the proportions of subset CD4+ and CD8 + T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4 + T cells between the medium-and low-dose groups. Interestingly, the Nanotechnology offers an efficient alternative for cancer diagnostics and tumor target treatment due to the unique properties of nanostructures, such as large surface-to-volume ratio, porous structure, embedded effect, and size effect, which have been recognized as offering potential promising applications in biomedical engineering. Much effort has been extended to the development of novel nanocomposites and biomaterials for DNA detection, 1 intracellular labeling, 2 drug carrier, 3 cancer targeting, 4 imaging, 5 and so on. Therefore, magnetic nanoparticles of Fe 3 O 4 (Fe 3 O 4 -MNPs) as a kind of biocompatible nanomaterial which is feasible to characterize and easily functionalize, may offer an exciting development toward developing an effective drug delivery system while biocompatible superparamagnetic particles like magnetite could be utilized in tissue-specific release of therapeutic agents and magnetic field assisted radionuclide therapy. [6][7][8][9] As a novel material, though we have already proved that Materials and methods experimental agentsExperimental agents were sourced from the following locations: RPMI1640 (Gibco Chemical Co., Carlsbad, CA, USA); Anti-CD3 (PE-Cy5), Anti-CD4, Anti-CD8 ( Pharmingen, San Diego, CA); Calf serum (Gibco Chemical Co); Enzyme-linked immunosorbent assay kit (Gibco, CA, USA); Con A (Sigma Chemical Co., St Louis, MO, USA). Preparation of Fe 3 O 4 -MNPsBased on our previous studies, 10-11 the synthesis of Fe 3 O 4 -MNPs was prepared by the electrochemical deposition under oxidizing conditions. Before being applied in the present experiment, the magnetite nanoparticles were well-distributed in RPMI-1640 medium freshly added with 10% heated-inactivated fetal bovine serum (FBS) using ultrasound treatment in order to obtain Fe 3 O 4 -MNPs colloidal suspension. Animals and animal careFemale an...

No abstract