Chlorophytes (which represent a clade within the Viridiplantae and a sister group of the Streptophyta) probably dominated marine export bioproductivity and played a key role in facilitating ecosystem complexity before the Mesozoic diversification of phototrophic eukaryotes such as diatoms, coccolithophorans, and dinoflagellates. Molecular clock and biomarker data indicate that chlorophytes diverged in the Mesoproterozoic or early Neoproterozoic, followed by their subsequent phylogenetic diversification, multicellular evolution, and ecological expansion in the late Neoproterozoic and Paleozoic. This model, however, has not been rigorously tested with paleontological data because of the scarcity of Proterozoic chlorophyte fossils. Here we report abundant millimeter-sized, multicellular, and morphologically differentiated macrofossils from ~1,000 Ma rocks. These fossils are described as Proterocladus antiquus new species and are interpreted as benthic siphonocladalean chlorophytes, suggesting that chlorophytes acquired macroscopic size, multicellularity, and cellular differentiation nearly a billion years ago, much earlier than previously thought.

The well-known debate on the nature and origin of intracellular inclusions (ICIs) in silicified microfossils from the early Neoproterozoic Bitter Springs Formation has recently been revived by reports of possible fossilized nuclei in phosphatized animal embryo-like fossils from the Ediacaran Doushantuo Formation of South China. The revisitation of this discussion prompted a critical and comprehensive investigation of ICIs in some of the oldest indisputable eukaryote microfossils-the ornamented acritarchs Dictyosphaera delicata and Shuiyousphaeridium macroreticulatum from the Paleoproterozoic Ruyang Group of North China-using a suite of characterization approaches: scanning electron microscopy (SEM), transmission electron microscopy (TEM), and focused ion beam scanning electron microscopy (FIB-SEM). Although the Ruyang acritarchs must have had nuclei when alive, our data suggest that their ICIs represent neither fossilized nuclei nor taphonomically condensed cytoplasm. We instead propose that these ICIs likely represent biologically contracted and consolidated eukaryotic protoplasts (the combination of the nucleus, surrounding cytoplasm, and plasma membrane). As opposed to degradational contraction of prokaryotic cells within a mucoidal sheath-a model proposed to explain the Bitter Springs ICIs-our model implies that protoplast condensation in the Ruyang acritarchs was an in vivo biologically programmed response to adverse conditions in preparation for encystment. While the discovery of bona fide nuclei in Paleoproterozoic acritarchs would be a substantial landmark in our understanding of eukaryote evolution, the various processes (such as degradational and biological condensation of protoplasts) capable of producing nuclei-mimicking structures require that interpretation of ICIs as fossilized nuclei be based on comprehensive investigations.

Abstract:Human activity intensity is a synthesis index for describing the effects and influences of human activities on land surface. This paper presents the concept of human activity intensity of land surface and construction land equivalent, builds an algorithm model for human activity intensity, and establishes a method for converting different land use/cover types into construction land equivalent as well. An application in China based on the land use data from 1984 to 2008 is also included. The results show that China's human activity intensity rose slowly before 2000, while rapidly after 2000. It experienced an increase from 7.63% in 1984 to 8.54% in 2008. It could be generally divided into five levels: Very High, High, Medium, Low, and Very Low, according to the human activity intensity at county level in 2008, which is rated by above 27%, 16%-27%, 10%-16%, 6%-10%, and below 6%. China's human activity intensity was spatially split into eastern and western parts by the line of Helan Mountains-Longmen Mountains-Jinghong. The eastern part was characterized by the levels of Very High, High, and Medium, and the levels of Low and Very Low were zonally distributed in the mountainous and hilly areas. In contrast, the western part was featured by the Low and Very Low levels, and the levels of Medium and High were scattered in Gansu Hexi Corridor, the east of Qinghai, and the northern and southern slopes of Tianshan Mountains in Xinjiang.