Context: Researchers have established that superior migration of the humeral head increases after fatigue of the rotator cuff muscles. In these studies, the investigators used imaging techniques to assess migration of the humeral head during statically held shoulder positions. Their results may not represent the amount of superior humeral head migration that occurs during dynamic arm elevation.Objective: To investigate the effect of rotator cuff fatigue on humeral head migration during dynamic concentric arm elevation (arm at the side [approximately 06] to 1356) in healthy individuals and to determine the test-retest reliability of digital fluoroscopic video for assessing glenohumeral migration.Design: Test-retest cohort study. Setting: Research laboratory.Patients or Other Participants: Twenty men (age 5 27.7 6 3.6 years, mass 5 81.5 6 11.8 kg) without shoulder disorders participated in this study.Intervention(s): Three digital fluoroscopic videos (2 prefatigue and 1 postfatigue) of arm elevation were collected at 30 Hz. The 2 prefatigue arm elevation trials were used to assess test-retest reliability with the arm at the side and at 456, 906, and 1356 of elevation. The prefatigue and postfatigue digital fluoroscopic videos were used to assess the effects of rotator cuff fatigue on glenohumeral migration. All measurements were taken in the right shoulder.Main Outcome Measure(s): The dependent measure was glenohumeral migration (in millimeters). We calculated the intraclass correlation coefficient and standard error of the measurement to assess the test-retest reliability. A 2 3 4 repeated-measures analysis of variance was used to assess the effects of fatigue on arm elevation at the 4 shoulder positions.Results: The test-retest reliability ranged from good to excellent (.77 to .92). Superior migration of the humeral head increased postfatigue (P , .001), regardless of angle.Conclusions: Digital fluoroscopic video assessment of shoulder kinematics provides a reliable tool for studying kinematics during arm elevation. Furthermore, superior migration of the humeral head during arm elevation increases with rotator cuff fatigue in individuals without shoulder dysfunction.Key Words: biomechanics, humeral head migration, imaging Key PointsN Superior migration of the humeral head increased during dynamic arm elevation after the supraspinatus, infraspinatus, and teres minor muscles of the rotator cuff were fatigued.N When used to analyze humeral head migration relative to the glenoid fossa, digital fluoroscopic video had good to excellent intrarater reliability.N Research is required to determine the influence of rotator cuff fatigue on glenohumeral migration in individuals with underlying shoulder disorders.

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