From the last few decades, there has been an increasing research interest in the value of lignocellulosic biomass. Lignoellulosic biomass is an inexpensive, renewable abundant and provides a unique natural resource for large-scale and cost-effective bio-energy collection. In addition, using lignocellulosic materials and other low-cost biomass can significantly reduce the cost of materials used for ethanol production. Therefore, in this background, the rapidly evolving tools of biotechnology can lower the conversion costs and also enhance a yield of target products. In this context, a biological processing presents a promising approach to converting lignocellulosic materials into energy-fuels. The present summarized review work begins with an overview on the physio-chemical features and composition of major agricultural biomass. The information is also given on the processing of agricultural biomass to produce industrially important enzymes, e.g., ligninases or cellulases. Cellulases provide a key opportunity for achieving tremendous benefits of biomass utilization.

In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35˚C.