No abstract

Purpose:To investigate the influence of targeted trypsin digestion and 16 hours compression loading on MR parameters and the mechanical and biochemical properties of bovine disc segments. Materials and Methods:Twenty-two 3-disc bovine coccygeal segments underwent compression loading for 16 hours after the nucleus pulposus (NP) of each disc was injected with a solution of trypsin or buffer. The properties of the NP and annulus fibrosus (AF) tissues of each disc were analyzed by quantitative MRI, biochemical tests, and confined compression tests. Results:Loading had a significant effect on the MR properties (T 1 , T 2 , T 1 , MTR, ADC) of both the NP and AF tissues. Loading had a greater effect on the MR parameters and biochemical composition of the NP than trypsin. In contrast, trypsin had a larger effect on the mechanical properties. Our data also indicated that localized trypsin injection predominantly affected the NP. T 1 was sensitive to loading and correlated with the water content of the NP and AF but not with their proteoglycan content. Conclusion:Our studies indicate that physiological loading is an important parameter to consider and that T 1 contributes new information in efforts to develop quantitative MRI as a noninvasive diagnostic tool to detect changes in early disc degeneration.

Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.