The major objective of the research is to investigate the in vitro antibacterial susceptibility to fatty acids contained in coconut oil on a collection of clinical isolates. especially, the chronic skin condition Atopic dermatitis (Atopic eczema). caused by gram-positive bacteria (Staphylococcus aureus). Clinical isolates are collected from Shar Hospitals, and the organisms pass through a typical biochemical examination evolution. While coconut oil is obtained using the centrifuge method to extract coconut oil (CE VCO). The sensitivity evaluation is conducted using the disc diffusion method. Lastly, use a measuring tool to estimate the inhibitory zones' diameter. In another hand, after analysis of the oil by GC-MASS, Coconut oil contained numerous chemical components and the highest amount is Oleic acid 54.62%, while the smallest amount was N-Hexadecanoic acid, N-Hexadecanoic acid, (Myristic acid), % 3.34. Sequentially, Heneicosane, 9-Octadecenoic acid (Z)-% 13.78, with 9,12-Octadecadienoic acid 11.23%, and Nonadecane and his isomers 5.77% percent. Coconut oil doesn't show any sensitivity and a high potential for antibacterial activity due to the high contents of medium and long-chain saturated and unsaturated fatty acids so This study recommends further studies should be done on the oil and its derivative both in vitro and in vivo for showing its mechanisms of actions.