Background: Wound infection is one of the health problems that are caused and aggravated by the invasion of pathogenic organisms. Objective: The objective of the study was to compare the bacteriological profiles including antimicrobial sensitivity patterns from wound swab isolates among hospital-acquired infection and community-acquired infection so that recommendations can be made for preventing resistance and empirical antibiotic treatment. Methodology: This retrospective study was conducted in the Department of Microbiology at Monno Medical College, Manikganj, Bangladesh during the period from June 2020 to July 2021 for a duration of one year. The patients attending the outpatient department were represented as community-acquired infection and patients admitted at least 48 hours were represented as hospital-acquired infection. All the samples were inoculated on blood agar and Mac Conkey agar media for 24 to 48 hours at 35 to 37°C. Organisms were identified by standard microbiological procedures. An antimicrobial susceptibility test was done for all isolated bacteria by the disc diffusion method. Results: A total number of 170 patients were recruited among them hospital-acquired infection was predominant at 96(56%) patients and community-acquired infection was at 87(49%) patients. About 126 (74.1%) yielded growth of different bacteria and culture-positive cases were found in 78 (61.9%) in the HAI group. The predominant isolate is Staphylococcus aureus majority were found in the HAI than CAI which was 36(57.1%) and 27(42.9%) respectively. Followed of Escherichia coli majority were found in the HAI than CAI which was 17(68.0%) and 8(32.0%) respectively. Streptococcus pyogenes were found 11(61.1%) of the HAI, Pseudomonas species majority were found in the HAI than CAI which was 10(62.5%) and 6(37.5%) respectively and Klebsiella species were found in the HAI 4 (100.0%). Staphylococcus aureus was found highly sensitive to sulphamethoxazole (100%), imipenem (79.4%), and gentamicin(76.2%). Escherichia coli was found highly sensitive to sulphamethoxazole (100%), ceftazidime (100%), and gentamicin (100%). Conclusion: Staphylococcus aureus was the most frequently isolated pathogen from wound swab among both HAI & CAI. Antibiotic sensitivity patterns of various isolates will guide for appropriate selection of antibiotics to reduce the spread of resistant bacteria against wound infection.  Bangladesh Journal of Medical Microbiology, July 2022;16(2):53-59

Background: Acinetobacter baumanii is responsible for nosocomial infections which are related to the biofilm forming capacity of this pathogen. Objective: The purpose of the present study was to detect biofilm formation in clinical isolates of Acinetobacter baumannii and to observe relationship between biofilm formations with its antimicrobial resistance. Methodology: This cross-sectional study was conducted in the Department of Microbiology of Dhaka Medical College and Hospital, Dhaka, Bangladesh from July 2015 to June 2016. Acinetobacter baumannii was isolated from different specimens and was identified and were screened for biofilm production by tissue culture plate method. Antimicrobial susceptibility test was done by disc diffusion method. Results: A total 300 samples were studied of which 26(8.7%) were Acinetobacter baumannii. From 26 isolated Acinetobacter baumannii, 16(61.5%) were biofilm producers. Biofilm producing Acinetobacter baumannii were 100% resistant to ceftriaxone, ceftazidime, amoxiclav, amikacin and ciprofloxacin. Resistance to imipenem, meropenem, cephotaxime, cefepime and gentamicin were also higher among biofilm producing Acinetobacter baumannii isolates than non-biofilm producers. Conclusions: In conclusion the ability of Acinetobacter baumannii forms biofilm and biofilm production has strong association with antimicrobial resistance. Bangladesh Journal of Infectious Diseases December 2021;8(2):82-86

Background: A multi-modality imaging approach plays a vital role for diagnosis however have a nonspecific appearance and need pathological confirmation to arrive at a specific diagnosis. Objective: Evaluation of the demographic profile and Clinical Presentation in orbital mass lesion among paediatric group would provide a general guideline to diagnose the nature of orbital mass lesion along with each histological variety. Methodology: This cross sectional study was conducted in the Department of Radiology and Imaging of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of January 2012 to December 2013 for duration of two year. The all patient below 18 years of age presented with suspected orbital mass and performed CT scan of orbit for diagnosis of the disease and also done histopathology after operation. Result: A total number of seventy cases were selected among them majority 25(35.7%) patients belonged to age ≤5 years and distribution of male to female ratio was 1.3:1.Clinical presentation of the patients showed that majority 52(74.1%) had decrease vision of eye and 24(34.3%) had proptosis. In this study a total of 42 benign cases was identified by histopathology among them 21.4% patients had dermoid and identified 28 malignant cases, among them 12.9% had retinoblastoma. Conclusion: Evaluation of CT scan findings in orbital mass lesion among paediatric group would provide a general guideline to diagnose the nature of mass lesion.

not available Bangladesh Journal of Infectious Diseases June 2021;8(1):1-2

Background: Seroprevalance of diphtheria antibody may vary in different socioeconomic people. Objective: The purpose of the present study was to determine the seroprevalence of diphtheria IgG antibody and to identify the relationship between diphtheria seroprevalence and several sociodemographic characteristics. Methodology: This cross-sectional study was conducted in the Department of Microbiology at Sylhet MAG Osmani Medical College, Sylhet, Bangladesh from July 2016 to June 2017 for duration of one year. Apparently healthy individuals were enrolled in this study as study population by following a set of inclusion and exclusion criteria. For laboratory procedure, anti-diphtheria antibody titer was measured by ELISA method. Result: A total number of 200 healthy individuals were recruited for this study. The mean serum IgG level was 0.23±0.26 IU/mL in participants of aged between 18 to 25 years which was much lesser in participants of aged between 26 to 32 years and 33 to 38 years group (p=0.001). Mean serum IgG level was 0.12±0.12 IU/mL in male participants. Majority participants have been come from lower middle class having mean serum IgG level of 0.10±0.21 IU/mL. Conclusion: In conclusion diphtheria IgG level is most commonly found among young male adult person in upper middle class socioecomonic condition. Journal of National Institute of Neurosciences Bangladesh, July 2021, Vol. 7, No. 2, pp. 156-160