A highly efficient strategy for recovery of genomic DNA from bone and tooth samples is presented by complete demineralization of the bone pieces or intact tooth with high concentration of EDTA followed by spin column treatment without the need of mechanical grinding or cryogenic method for pulverizing the samples. The DNA yield was between 8 and 12 ng/μl from approximately 1 -2 g of the starting material. Completed DNA profiles were obtained from of all the bones (52) and tooth (270) samples received from the unidentified victims from a recent building collapse, the Rana Plaza disaster in Dhaka, Bangladesh.

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Background: Hepatitis B virus (HBV) infection is a serious global public health problem as well as in Bangladesh. The most common liver disease in Bangladesh is viral hepatitis. Bangladesh is an intermediate endemic zone for hepatitis B virus infection. Research shows that about 10 million people in Bangladesh have been suffering from hepatitis B.Methods: The research was a cross-sectional survey and appropriate statistical formula was used to select 341 respondents randomly. The data were collected through a pre-designed, pre-structured questionnaire, which was distributed among the students. All the data was analysed using Microsoft Excel 2013.Results: Most of the respondent 95% knew about hepatitis B and about 87% knew that it is a viral infection. About 69% students respond that hepatitis B can increase liver cancer. Among the respondents, 76% supported that hepatitis B can be transmitted by blood transfusion. Most of the students 82% supported blood transfusion as a causative factor and 67% informed that jaundice is the major sign and symptoms of hepatitis B. More than half of the students 53% reported that they were vaccinated for hepatitis B. The major reasons for not taking vaccination were lack of free time and lack of feeling of necessity for vaccination.Conclusions: The overall knowledge and vaccination status on hepatitis B virus was not satisfactory. Different types of seminars and campaigns on hepatitis B, arranged by university will help to increase the student’s level of knowledge on hepatitis B.

A case study was conducted to evaluate the significance of adding lineage markers for a complex kinship analysis. Additional genetic information may increase likelihood ratio values for true relationships in a pedigree, while reducing the chance of identifying false relationships. It is very difficult to establish a relationship between siblings by the statistical evaluation with autosomal STR markers. The clear discrimination of true versus false relationships is important for complex kinship cases, such as sibling testing, deficient paternity testing, missing person’s identification as well as immigration testing with the help of forensic DNA technology. This case study represents a false parent-child relation or full sibling, one individual is male and other individual is female, where matches were found at least one allele at all 23 tested autosomal STR loci with Combined Likelihood is 75,446,930.822 for Parent-Child and 284,041.488 for Half-Sibling indicates that 266 times more likely parent-child than half-sibling. In order to reach an accurate conclusion, we further included one sister of the putative brother and mother of the putative sister and these four individuals DNA samples were analyzed with X-chromosome STR kits, showing mismatches between claimed siblings in eight loci out of twelve loci and confirm that they are not parent-child. Finally we confirm that they are half-sibling and they are come from same father but different mother. This case emphasizes the necessity of lineage markers like X chromosome or Y chromosome DNA data for interpreting critical sibling cases where one of the siblings is female and their father is dead. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 148-151

Type 2 diabetes mellitus is one of the deadliest disease in Bangladesh as its complications are enormous and life threatening. This study demonstrates the prevalence of the complications of type 2 diabetes among the population of Bangladesh. Data of 1000 subjects admitted into BIRDEM General Hospital from May 2015 to April 2016 were taken into consideration. Significantly, it was found that 89% patients had fasting blood glucose (FBG) level greater than 7 mmol/l and 70.8% patients had glycosylated hemoglobin (HbA1c) level greater than 8%. As a consequence, the total population were affected by diverse health complications including lung, liver, cardiovascular, kidney, brain, oral, eye, thyroid, hearing, skin, and so on. Males and females were suffered from these complications without biasedness.