Sweet marjoram (Majorana hortensis) is an important aromatic herbal plant that has long been used and well managed in the traditional and general medical, pharmaceutical, food, cosmetic, and perfume industries. Thus, the increase in its productivity appears to be of great value since there is a large number of bioactive secondary metabolites as well as an increase in the demand in domestic or foreign markets. The purpose of this study is the possibility of promoting the sustainable development of marjoram in the framework of organic farming through gamma irradiation, chitosan and yeast. Field experiments were conducted in a factorial split-plot design with three iterations over two consecutive seasons (2019 and 2020). The main plot is an abiotic elicitor (15 Gy gamma irradiation), two biotic elicitors 500 ppm chitosan, 0.5% yeast, and a non-elicitor (as control), while in the sub-main plot, there were two organic fertilizers, water extract of moringa 20 g/m2 dry leaves, 20 g/m2 fulvic acid, and 20 g/m2 (NPK); the latter is a traditional agrochemical. Statistical analysis of all characteristics of production and quality of biomass and biologically active secondary metabolites revealed that the use of organic fertilizers helped in increasing the yield of marjoram, both qualitatively and quantitatively, and significantly outperformed the chemical fertilizer. The experiment enhances the comprehensive and integrated development of marjoram under organic cultivation and achieves a promising alternative to traditional cultivation without the use of microbicides and/or agrochemical pesticides.

Artemisia plants process potential bioactive secondary metabolites such as artemisinin and essential oil. They are useful in controlling potential pests and microbes and have a therapeutic effect. The commercial production of artemisinin and essential oil is limited to regarding the worldwide demand. Urgent attempts must be undertaken to improve the production of bioactive secondary metabolites. The objectives of this experiment were to increase the production and improve the quality of bioactive secondary metabolites in order to limit the use of agrochemicals. Therefore, a field experiment was conducted during the 2018 and 2019 seasons. Treatments included three elicitations (gamma irradiation, nano-selenium, and chitosan) and three kinds of fertilizers (NPK, Moringa leaf extract, and humic acid). The experiment was conducted as a factorial with a completely randomized block. The designs and treatments were arranged in a split–split plot with three replicates. A single application of elicitors showed that chitosan > nano-selenium, chitosan > gamma irradiation, and Moringa > humic acid > NPK in plants’ artemisinin and essential oil content, while the interaction showed a significant synergistic relationship between elicitors and the fertilizers in enhancing the quantity and quality of artemisinin and essential oil of Artemisia plants. Without using any pesticides, there was no infection that appeared in Artemisia plants, this could be due to the enhancement of bioactive secondary metabolite production.