ORIGINAL PROF-3398ABSTRACT… Pancytopenia refers to a reduction in all the three cellular elements of blood. Pancytopenia is a common but important hematological problem encountered in routine clinical Practice. It may be a presenting feature of many underlying serious and life threatening illnesses. Objective: Present study was planned to appraise various clinico-etiological factors underlining Pancytopenia. Study Design: Cross-sectional study. Setting: Hematology Department of Allama Iqbal Medical College/ Jinnah Hospital Lahore (AIMC&JHL). Period: July 2011 to Jun 2015. Materials and Methods: Two hundred and forty four patients with Pancytopenia were included in the study. Complete blood count, bone marrow aspirations and trephine biopsies were performed after clinical history and physical exam. Data were analyzed by using SPSS 20.0 version. Results: Out of total 244 Pancytopenia cases, hypoplastic bone marrow 66 (27.04%) and Megaloblastic anemia 49 (20.08%) were the most common etiological factors with male predominance. Fever and pallor were striking presenting clinical features. Conclusion:We conclude that Pancytopenia is a common problem with an exhaustive list of differential diagnosis, some of which are easily treatable and reversible. Therefore early detection with optimal diagnostic approach to Pancytopenia is required for better management of patients. Key words:Pancytopenia, Aplastic anemia, Megaloblastic anemia, Bone marrow.