Background: Eight inter-simple sequence repeats (ISSR), 14 Start codon-targeted (SCoT), and eleven sequence-related amplified polymorphisms were used to determine genetic diversity among five basil species (Ocimum tenuiflorum, Ocimum kilimandscharicum Guerk, Ocimum basilicum L., Ocimum americanum, and Ocimum basilicum var. minimum). Results: All of the markers' PCR products can be used to discriminate between the different basil species. The ISSR has the highest polymorphism percentage (73.1%), followed by SCoT (57.2%) and SRAP (55.6%). According to the combined data from the three markers, the basil species used were clustered into four groups, each with extremely excellent coordination between the similarity matrix and dendrograms. Species-specific markers have been identified. The SCoT analysis generates the highest number of species-specific marker (17) compared with ISSR and SRAP analysis which generated 14 and 13 markers respectively. Conclusions: These markers can be considered as a useful marker to improve Ocimum characteristics.

Background: Eight inter-simple sequence repeats (ISSR), 14 Start codon-targeted (SCoT), and eleven sequence-related amplified polymorphisms were used to determine genetic diversity among five basil species (Ocimum tenuiflorum, Ocimum kilimandscharicum Guerk, Ocimum basilicum L., Ocimum americanum, and Ocimum basilicum var. minimum). Results: All of the markers' PCR products can be used to discriminate between the different basil species. The ISSR has the highest polymorphism percentage (73.1%), followed by SCoT (57.2%) and SRAP (55.6%). According to the combined data from the three markers, the basil species used were clustered into four groups, each with extremely excellent coordination between the similarity matrix and dendrograms. Species-specific markers have been identified. The SCoT analysis generates the highest number of species-specific marker (17) compared with ISSR and SRAP analysis which generated 14 and 13 markers respectively. Conclusions: These markers can be considered as a useful marker to improve Ocimum characteristics.