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Plants have been explored extensively all over the globe in quest of a novel bioactive compound that could a good therapeutic candidate treating infectious diseases especially against drug resistant microbes. Qualitative phytochemical analyses of Carica papaya leaf extract reveal that except steroids and tannins all the possible phytochemical constituents including carbohydrates, proteins, anthraquinones, flavonoids, saponins, cardiac glycosides and alkaloids were present. Two ways of Carica papaya leaf extract preparations i.e crushed and boiled were tested for their antifungal activity against 6 saprophytic fungi Penicillium sp, Aspergilus flavus, Aspergillus niger, Fusarium sp, Rhizopus and Helminthosporum, 5 dermatophytic fungi Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and 6 yeasts including Candida albicans, Candida albicans ATCC 0383, Saccharomyces cerevisiae, Candida galbrata, Candida tropicalis, Candida kruzei. The activity was found against majority of fungi but was much better in case of crushed leaf extract.

Wild plants have always held economic, nutritional and medicinal value for human beings. Present work is the study of local information of some wild plants being used for remedial purposes in District Muzaffarabad, Azad Jammu and Kashmir, Pakistan. The indigenous knowledge of local conventional uses was collected through survey and personal interviews during field trips. A total of 50 plant species were identified by taxonomic description using field guides and locally by medicinal knowledge of people living in the area. About 150 informers were interviewed randomly to record local names and ethno-medicinal uses of different plant species.

The present investigation focuses on the floristic composition in wet temperate forests of Pakistan. This area was selected as it has variation and diversity i.e. downward the tropical thorn forests and upward the alpine meadows. Wet temperate forest has high diversity of vulnerable plant species. It is situated in the Galiat Forest plants division of Abbottabad between 34•-1' to 34•-3.8' N latitude and 73•-2222.8' to 73•-27.1' E longitude over an area of about 1684 hectares. Five hierarchy levels of classification of 27 sites were analyzed by (DCA). Four plant associations were recognized on the basis of cluster analysis. Deterended Correspondence Analysis (DCA) and Correlation Coefficient to detect the relationship between altitude and some environmental factors with the composition and structure of plants communities. The major families were Rosaceae, Pteridaceae, Pinaceae, Lamiaceae and Asteraceae.