This paper presents the chromosome numbers (2n) for 13 alien species (from the families Apocynaceae, Asteraceae, Brassicaceae, Fabaceae, Lamiaceae, Polygonaceae, Solanaceae) in the Novosibirsk Region. For Vincetoxicum hirundinaria Medik. (2n = 22) and Sisymbrium altissimum L. (2n = 14), chromosome numbers were determined for the first time on the material from Russia; for Betonica officinalis L. (2n = 16) – from Asian Russia; for Stachys annua (L.) L. (2n = 34) – from West Siberia. Chromosome numbers on the material from the Novosibirsk Region were revealed for the first time for Cosmos bipinnatus Cav. (2n = 24), Inula helenium L. (2n = 20), Sonchus oleraceus L. (2n = 18), Medicago sativa subsp. varia(Martyn) Arcang. (2n = 32), Rumex obtusifolius L. (2n = 20) and Solanum nigrum L. (2n = 48). For all the species studied, brief notes on their general distribution and dispersal in the Novosibirsk Region are provided, along with literature data on chromosome numbers from other regions of Russia.