The current investigation aimed to present an overview of the conservation of biological diversity of rare and endangered plant species. Methods of biodiversity conservation as well as several overview recommendations for the preservation of various rare species have been considered. An overview of the taxa included in the red book has been presented on the example of the Russian Federation. Global and local codes and classifiers of plant rarity were also presented. Future prospects for the conservation of biological diversity and the creation and development of bioresource collections have been considered.

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A dynamic subcritical water extraction method of benzo [a]pyrene from soils is under consideration. The optimum conditions for benzo [a]pyrene extraction from soil are described including the soil treatment by subcritical water at 250 °C and 100 atm for 30 min. The effectiveness of developed method was determined using the matrix spiking recovery technique. A comparative analysis was made to evaluate the results of benzo[a]pyrene extraction from soils using the subcritical water and organic solvents. The advantages of the subcritical water extraction involve the use of ecologically friendly solvent, a shorter time for the analysis and a higher amount of benzo[a]pyrene extracted from soil (96 %). The influence of subcritical water extraction on soil properties was measured the investigation of the processes occurring within soil under the influence the high temperature and pressure. Under appropriate conditions of the experiment there is the destruction of the soil organic matter while the composition of the soil mineral fraction remains practically unchanged.

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