Summary Athletes feel greater need for macro-and microelements as, due to great training loads, the body loses salts. Normal course of vital processes is often hindered not only by the lack of minerals into two groups. The first (I) group (male, n=17) consisted of Greco-Roman wrestlers. The second group (II) (women, n=9) -volleyball players. Control groups consisted of 25 students. The first (I) control group (men, n=15) and second (II) control group (women, n=10) consisted of students, who were not involved in sports activities. For establishing the concentration of macro-and microelements in hair, x-ray fluorescence method was applied using CEP-1 ELVAX (MP.MN 3730-2011) device. X-ray fluorescence method is a perspective and informative way for establishing concentration of macro-and