Please cite this article as: Lević, S., Pajić Lijaković, I., Đorđević, V., Rac, V., Rakić, V., Šolević Knudsen, T., Pavlović, V., Bugarski, B., Nedović, V., Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion, Food Hydrocolloids (2014), Abstract 17 18 In this study, calcium alginate beads immobilizing D-limonene (solid systems) have been 19 manufactured starting from emulsions of this flavor in sodium alginate (liquid systems). The 20 effects of alginate concentration (0.02 and 0.03 g/mL) and flavor content (5 and 10 %w/w) on 21 viscosity, conductivity and stability of emulsions were investigated. The flavor droplets in 22 emulsions are bigger as polymer solution is more concentrated and contains more of the flavour.23When emulsions have been subjected to electrostatic extrusion and upon Na + -Ca 2+ ion exchange, 24 smaller (~960 to ~1450 µm) and less spherical beads were obtained (sphericity factor 0.003 to 25 M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 2 0.21) compared to beads produced by simple dripping technique (without electrostatic field). 26When wet beads were air dried, they shrunk less if they had higher content of the flavour. Novel 27 mathematical model describing swelling kinetics of dried beads is developed. In this work, D-28 limonene was efficiently immobilized within Ca-alginate beads (immobilization efficiency ~50 29 to ~77%) and its thermal stability was confirmed by TG/MS analysis. 30 31

Abandoned nonferrous metal(loid) tailings sites are anthropogenic, and represent unique and extreme ecological niches for microbial communities. Tailings contain elevated and toxic content of metal(loid)s that had negative effects on local human health and regional ecosystems. Microbial communities in these typical tailings undergoing natural attenuation are often very poorly examined. The diversity and inferred functions of bacterial communities were examined at seven nonferrous metal(loid) tailings sites in Guangxi (China), which were abandoned between 3 and 31 years ago. The acidity of the tailings sites rose over years of site inactivity. Desulfurivibrio, which were always coupled with sulfur/sulfide oxidation to dissimilate the reduction of nitrate/nitrite, were specific in tailings with 3 years abandonment. However, genus beneficial to plant growth (Rhizobium), and iron/sulfur-oxidizing bacteria and metal(loid)-related genera (Acidiferrobacter and Acidithiobacillus) were specific within tailings abandoned for 23 years or more. The increased abundance of acid-generating iron/sulfur-oxidizing and metal(loid)-related bacteria and specific bacterial communities during the natural attenuation could provide new insights for understanding microbial ecosystem functioning in mine tailings. OTUs related to Sulfuriferula, Bacillus, Sulfurifustis, Gaiella, and Thiobacillus genera were the main contributors differentiating the bacterial communities between the different tailing sites.Multiple correlation analyses between bacterial communities and geochemical parameters indicated that pH, TOC, TN, As, Pb, and Cu were the main drivers influencing the bacterial community structures. PICRUSt functional exploration revealed that the main functions were related to DNA repair and recombination, important functions for bacterial adaptation to cope with the multi-contamination of tailings. Such information provides new insights to guide future metagenomic studies for the identification of key functions beyond metal-transformation/resistance. As well, our results offers novel outlooks for the

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