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The fuel butanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as carboxylic acids, phenolic compounds and furans formed during hydrolysis. The main objective of this work is to evaluate the biological detoxification method of lignocellulosic hydrolysates when two forms of specially adapted activated sludge are used for improved biobutanol production. We investigated the removal of toxic substances from an enzymatic hydrolysate of miscanthus cellulose and an acid hydrolysate of spruce by specially adapted activated sludge of the urban wastewater treatment plants and by activated sludge of the pig farm wastewater treatment plants. Activated sludge treatment removed 98 % and 99 % of 5-hydroxymethylfurfural (5-HMF) and furfural from the hydrolysate respectively. Fermentation of treated hydrolysates by Clostridium acetobutylicum ATCC 824 was successful. Control experiments on fermentation of untreated hydrolysates showed a complete absence of fermentation

Realization of an inexpensive and effective detoxification method of lignocellulosic hydrolysates is a prerequisite for reducing the cost of biofuels production. We suggest a cost-effective waste-free method of hydrolysates purification from acetone-butanol inhibitors, which is based on the use of specially adapted activated sludge. We conducted the detoxification of an enzymatic hydrolysate of miscanthus cellulose and an acid hydrolysate of spruce by specially adapted activated sludge of the urban wastewater treatment plants. As a result of purification of the spruce hydrolysate, the concentration of 5-HMF has decreased from 3.06 g/L to 0.0012 g/L, furfural -from 1.3 g/L to 0.0009 g/L. The concentrations of 5-HMF have reduced from 1.03 g/L to 0.0013 g/L and the concentrations of furfural have decreased from 0.71 g/L to 0.0008 g/L. We showed the effectiveness of the method by fermentation of the medium based on treated or untreated hydrolysate by Clostridium acetobutylicum ATCC 824. The obtained results can be valuable not only for the production of biobutanol, but in the technologies for bioethanol production.

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