The attachment of salivary bacteria initiates the formation of dental plaque to the acquired pellicle covering the tooth surface. This Research aimed to investigate the prevalence and antibiogram study of isolated bacteria from Tasmia dental care Saidpur, Bangladesh. In this study, we selected 200 patients (aged 20-60 years) to isolate desired isolates. 16S rRNA gene sequencing techniques were applied for molecular confirmation of isolated bacteria. 55.83% of patients exhibited molar caries, 27.5% between molar and premolar, 12.5% at the incisor, and 4.17% at the cervical border. Dental caries was (P < 0.001) prevalent in age groups 31-40 (82.86%), 41-50 (72.22%), and above 50 (34.29%), respectively. In contrast to patients from high-and middle-income households, those from low-income families had a considerably higher prevalence of dental caries (79.78%) (P < 0.001). Dental caries was observed to be substantially (P < 0.001) more prevalent among the illiterate (84.0%) than among the educated (25.0%) or the very worried (8.33%). Staphylococcus spp. (33.33%), E. coli (25.0%), Streptococcus spp. (20.0%), Acromobacter xylosoxidans (16.67%) and Pseudomonas spp. (5.0%) were the most often isolated bacteria. Acromobacter_ xylosoxidans_strain_LMG_1863 was identified with 144bp in this Research. A. xylosoxidans, and E. coli were resistant to over six antibiotics. At the same time, all Pseudomonas spp.were resistant except for Ciprofloxacin and colistin. Staphylococcus spp. and Streptococcus spp. were also resistant to more than five antibiotic discs. Triclosan and fluoride-containing antibiotic-free toothpaste can be the best preventive methods for cavities.