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Irrational use of antimicrobials is a major problem worldwide. The comprehensiveness of the existing legislation and veterinary pharmaceutical regulatory system has been critically evaluated and its practical implementation was assessed in this study. A cross-sectional survey study, and an in-depth interview of key informants involving the layer and pig farm owners/managers of farms located in Chiang Mai, Lamphun and Chonburi Provinces were conducted. The Thai FDA is responsible for pre-marketing and authorizes relevant officials of DLD to enforce drug acts related to the post-marketing of veterinary drugs/biologics. These existing legislations and regulations were comprehensive enough to cover all areas of pharmaceutical activities developed to protect the health of the public and animals in the country; however, the enforcement of these rules may not be properly enforced or may be ineffectively executed. Rules regarding veterinary pharmaceuticals being distributed throughout the country have not been clearly stated. Farmers can easily access veterinary pharmaceuticals, and veterinarian prescriptions were not needed. Additionally, the relevant benefits were among the most important reasons for choosing to use antimicrobials on farms, along with a lack of availability of better alternative products for the treatment of infections. Intensive legislation and regulation enforcement were considered to be the most effective means of reducing antimicrobial usage and abuse in Thailand.

Mycoplasma gallisepticum (MG) is one of the most economically important pathogens worldwide. MG affects the respiratory system and impairs growth performance in poultry. In developing countries, the most widely used technique to identify MG is the conventional PCR assay. In this study, 24 MG isolates collected from Thailand farms with unvaccinated chickens during 2002–2020 were characterized by gene-targeted sequencing (GTS), followed by phylogenetic analysis using unweighted pair group method with arithmetic mean. These 24 Thai MG isolates differed from vaccine strains, including the F, ts-11 and 6/85 strains. One isolate showed 99.5–100% genetic similarity to the F strain with 4 partial gene analyses. This result may have been due to contamination from vaccinated flocks because the F strain is the most commonly used vaccine strain in Thailand. However, the GTS analysis using the partial MG genes in this study showed that the isolates could be grouped into different patterns based on individual gene sequences. The phylogenetic analysis of partial mgc2, gapA, pvpA and lp gene sequences classified the Thai MG isolates into 7, 11, 7 and 2 groups, respectively. In conclusion, at least 2 partial MG genes, especially partial gapA and mgc2 genes, are needed to differentiate MG isolates.

Human salmonellosis is a major public health problem worldwide. Infections can pass to humans by contact with contaminated substances in the food chain. This study aimed to determine the prevalence and contamination levels of Salmonella isolated from pork, chicken and beef sold in different types of retail stores in Chiang Mai and Lamphun provinces and to investigate the genetic relatedness among Salmonella isolates in food chains in that area. A total of 360 meat samples from supermarkets, mini‐grocery stores and fresh markets were obtained. Salmonella Rissen and S. Weltevreden were found in all meat sample types and in human cases. The overall prevalence of Salmonella in the chicken, pork and beef samples was 34.17%, 32.50% and 3.33%, respectively. Quantitatively, Salmonella contamination was highest in pork (1.24 log10MPN/g), followed by chicken (1.08 log10MPN/g), and beef (0.75 log10MPN/g). The highest frequency of Salmonella contamination was found at the fresh markets (85.71%), whereas the highest quantity of contamination level was from mini‐grocery stores (1.27 log10MPN/g). The rep‐PCR analysis results revealed that some of the Salmonella from meat samples and human cases were identical clones.

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