The estimation of dominance effects requires the availability of direct phenotypes, i.e., genotypes and phenotypes in the same individuals. In dairy cattle, classical QTL mapping approaches are, however, relying on genotyped sires and daughter-based phenotypes like breeding values. Thus, dominance effects cannot be estimated. The number of dairy bulls genotyped for dense genome-wide marker panels is steadily increasing in the context of genomic selection schemes. The availability of genotyped cows is, however, limited. Within the current study, the genotypes of male ancestors were applied to the calculation of genotype probabilities in cows. Together with the cows' phenotypes, these probabilities were used to estimate dominance effects on a genome-wide scale. The impact of sample size, the depth of pedigree used in deriving genotype probabilities, the linkage disequilibrium between QTL and marker, the fraction of variance explained by the QTL, and the degree of dominance on the power to detect dominance were analyzed in simulation studies. The effect of relatedness among animals on the specificity of detection was addressed. Furthermore, the approach was applied to a real data set comprising 470,000 Holstein cows. To account for relatedness between animals a mixedmodel two-step approach was used to adjust phenotypes based on an additive genetic relationship matrix. Thereby, considerable dominance effects were identified for important milk production traits. The approach might serve as a powerful tool to dissect the genetic architecture of performance and functional traits in dairy cattle. IN the context of genomic selection in dairy cattle, an abundance of bulls has been genotyped by applying genomewide dense marker panels. In 2010, the European reference population comprised .17,000 bulls representing .20 million daughters (Lund et al. 2010;Liu et al. 2011). In addition to their utilization in genomic prediction, these data are extensively used in genome-wide association studies to unravel the genetic factors affecting performance and functional traits. The expression of these traits is naturally limited to female individuals and thus, the phenotypes used in association studies are usually breeding values of sires based on performance data of many daughters. Such a structure of data allows only the estimation of allele substitution effects. There is no direct possibility to distinguish between additive and dominance effects. For the detection of these allelic interactions, genotypes and phenotypes had to be known in the same individuals. Compared to the bulls, the availability of genotype data for cows is limited. With the increasing number of genotyped bulls, genotypes of male ancestors become available for many cows, enabling the derivation of genotype probabilities. Within the current study, these probabilities were converted to additive and dominance coefficients suitable for regression analysis analogous to the procedures commonly applied to QTL mapping in resource populations (Haley and Knott 199...

The distal part of SSC7, which contains the ortholog to the ovine region encompassing the callipyge locus, was analyzed in a Piétrain F(2) resource population comprising more than 2,700 offspring. The aim of the study was to map QTL affecting carcass traits comparable with the callipyge phenotype in sheep. We applied an interval mapping approach using 14 microsatellite markers and detected 3 QTL with small effects. The first QTL affects fat thickness on the middle of the back, with no detectable impact on fat sizes at other places on the back, whereas the second QTL affects side fat thickness. The third QTL influences the ham weight and shows a clear parent-of-origin-dependent effect in the form of maternal polar overdominance. It is not located at the position of the imprinting cluster including the callipyge locus, but 7 cM proximal.

The aim of this study was to test for the existence of causative genetic variation affecting body composition traits within or adjacent to the porcine insulin-like growth factor 2 (IGF2) gene beyond the known IGF2-intron3-G3072A mutation. A focussed quantitative trait loci analysis using four microsatellite markers within the telomeric region of porcine chromosome 2p was conducted in a large resource population comprising 2741 F2 offspring. The analysis of two subsets of animals that were alternatively homozygous for the in3G3072A mutation provides evidence for additional genetic variation significantly contributing to the overall quantitative trait nucleotide variance within our population.