The food composition of the Four-horned Antelope Tetracerus quadricornis was studied in the Churia Hills of Nepal during summer, monsoon and the winter seasons of 2012–2013.  Microhistological technique was used to determine the diet.  The Four-horned Antelope was found to be a mixed feeder feeding on trees, shrubs, forbs, grasses and climbers.  Trees and shrubs contribute the major percentage of diet in all the three seasons.  The Gramineae family is consumed in highest proportion.  Mitragyna parvifolia, Bridelia retusa, Bambusa vulgaris, Hymenodictyon sp. and Ziziphus mauritiana are major tree species while Barleria cristata, Pogostemon benghalensis, Achyranthes sp., Clerodendrum viscosum are among shrubs.  Ageratum conyzoides and Blumea virens are the main forbs Eulaliopsis binata and Imperata cylindrica are the principal grass species.  Climber Trachelospermum lucidum is consumed in a small proportion.  Grasses in monsoon were consumed distinctly at a higher percentage than during the other two seasons.  The Four-horned Antelopes are concentrated feeders and browsers with a generalized feeding strategy. Similar studies need to be conducted in other landscapes and with sympatric and potential competitor species to understand its niche overlaps and degree of competition.

Traditional knowledge on the use of animal products to maintain human health is important since time immemorial. Although a few studies reported food and medicinal values of different animals, a comprehensive ethno-medicinal study of vertebrates in Nepal is still lacking. Thus, present study is aimed at documenting the ethno-medicinal knowledge related to vertebrate fauna among different ethnic communities in the Chitwan-Annapurna Landscape, central Nepal. Data was collected by using semi-structured questionnaires and analyzed by using Use Value (UV), Informant Consensus Factor (ICF) and Fidelity level (FL). Results showed a total of 58 (53 wild and 5 domestic) species of vertebrate animals. They were used to treat 62 types human ailments. Four animals were also used for veterinary diseases and agriculture benefits. The most widely used species was Felis chaus (UV = 0.25) with 3 use-reports by 10 informants. Cardiovascular and dental problems had the highest ICF value (0.974) with cardiovascular problems having 351 use-reports for 10 animal species and dental problems having 77 use-reports for 3 animal species. The least ICF was found in ophthalmological problems (ICF = 0.833, use reports = 7 for 2 species). We concluded that the different animals were an important part of traditional medicine for the local people living in the Chitwan-Annapurna Landscape. However, the majority of animals and most likely to be threatened due to their uses. The present documented ethnozoological knowledge can be used in conservation and management of vertebrates so that they could be protected for future generations.

Estimating cause specific leopard (Panthera pardus) mortality is critical to their conservation. This paper examined leopard death reports during 2006-2013 in order to estimate cause-specific mortality, identify conservation issues related to leopard mortality and provide recommendations for reducing human-caused mortality in Nepal. Data revealed that the leopards in the human dominated landscape are susceptible to variation in survival caused by human induced mortality (65%), with retaliation (31%) and lethal control (20%) of declared problem leopard as a significant part. Elevated human induced mortality can cause large scale stochasticity influencing population dynamics of leopard. The conservation of leopards needs to acknowledge strategies to limit retaliatory killings and lethal control in the plans, while addressing its conflicts with human. Efforts to reduce human-caused mortality should focus on reducing poaching and deaths from human-leopards conflicts.

To identify the house mice collected in Pokhara and Lumbini of Nepal at the subspecies level, morphological and molecular analyses were carried out. Morphologically, two populations collected in Pokhara and Lumbini were distinguished by fur colour, but there was no significant difference in external measurements (p > .05). The phylogenetic analysis results revealed that the haplotypes sequences of mitochondrial DNA (mtDNA) Cytochrome B (CytB) gene distinguished into two distinct clades on a phylogenetic tree representing two subspecies, Mus musculus bactrianus and M. m. castaneus in Pokhara and Lumbini, respectively. In Nepal, the subspecies M. m. bactrianus was not reported before this study. These findings concluded that at least two subspecies, M. m. bactrianus and M. m. castaneus currently exist in Nepal. We estimated that these two subspecies could have introduced together with human migration, while further study is required to understand their evolutionary history and current distribution.

Factors affecting diversity and distribution of globally threatened birds were studied by dividing Chitwan National Park (CNP) into five study blocks consisting of 17 birding routes.  CNP provides major habitats for the feeding and breeding of a large number of migratory birds from many parts of the globe and also plays a vital role in the conservation of threatened species.  We recorded a total of 437 individuals of globally threatened birds belonging to 19 species of nine families and eight orders.  There was considerable variation (F=2.94, df=44.43, p=0.05) in species diversity of threatened birds in different study blocks: the highest diversity was in Block E (Pithauli, Amaltari, and Narayani Island area; H=2.108), followed by Block C (Kasara to Sukibhar area; H=2.047), Block B (Barandabhar Corridor Forest; H=2.033), Block A (Khagendra Malli, Kathar, Sauraha to Old Padampur; H=1.744), and Block D with the least diversity (Madi area; H=1.69).  The higher dominance index was found in blocks A (D=0.2407) and D (0.2361) compared to other blocks.  The lower diversity of threatened birds was reported in those blocks (A & D) located nearer to human settlements that experienced higher disturbance.  Presence of livestock and people caused significantly negative effects on species richness and abundance of threatened birds, mainly in Block A.  Distance from roads and villages also had a negative effect on the diversity and abundance of most of the threatened birds.  This study suggests that human disturbance caused a significantly negative impact on the presence, distribution, diversity, and abundance of threatened birds in CNP and adjoining areas.