No abstract

We investigated whether the surface-linked liposomal peptide was applicable to a vaccine based on cytotoxic T lymphocytes (CTLs) against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV). We first identified four HLA-A*0201-restricted CTL epitopes derived from SARS-CoV using HLA-A*0201 transgenic mice and recombinant adenovirus expressing predicted epitopes. These peptides were coupled to the surface of liposomes, and inoculated into mice. Two of the liposomal peptides were effective for peptide-specific CTL induction, and one of them was efficient for the clearance of vaccinia virus expressing epitopes of SARS-CoV, suggesting that the surface-linked liposomal peptide might offer an effective CTL-based vaccine against SARS.

ABSTRACT. Microscopic observation of air-hydrate crystals was carried out using 34 deep ice-core samples retrieved at Vostok Station, Antarctica. Samples were obtained from depths between 1050 and 2542 m, which correspond to Wisconsin/ Sangamon/Illinoian ice. It was found that the volume and number of air-hydrate varied with the climatic changes. The volume concentration of air-hydrate in the interglacial ice was about 30% larger than that in the glacial ice. In the interglacial ice, the number concentration of air-hydrate was about a half and the mean volume of air-hydrate was nearly three times larger than that in the glacial-a;e ice. The air-hydrate crystals were found to grow in the ice sheet, about 6.7 x 10-1 cm 3 year-I, in compensation for the disappearance of smaller ones. The volume concentration of air-hydrate was related to the total gas content by a geometrical equation with a proportional parameter a . The mean value of a below 1250 m, where no air bubbles were found, was about 0.79. This coincided with an experimentally determined value of the crystalline site occupancy of the air-hydrate in a 1500 m core obtained at Dye 3, Greenland (Hondoh and others, 1990). In the depth profile of calculated a for many samples, a in the interglacial ice was about 30% smaller than that in the glacial-age ice.

No abstract

We have previously demonstrated that liposomes with differential lipid components display differential adjuvant effects when Ags are chemically coupled to their surfaces. In the present study, Ag presentation of liposome-coupled OVA was investigated in vitro, and it was found that OVA coupled to liposomes made using unsaturated fatty acid was presented to both CD4+ and CD8+ T cells, whereas OVA coupled to liposomes made using saturated fatty acid was presented only to CD4+ T cells. Confocal laser scanning microscopic analysis demonstrated that a portion of the OVA coupled to liposomes made using unsaturated, but not saturated fatty acid, received processing beyond the MHC class II compartment, suggesting that the degradation of OVA might occur in the cytosol, and that the peptides generated in this manner would be presented to CD8+ T cells via MHC class I. The ability to induce cross-presentation of an Ag coupled to liposomes consisting of unsaturated fatty acid was further confirmed by in vivo induction of CTL and by the induction of tumor eradication in mice; E.G7 tumors in mice that received combined inoculation with OVA257–264-liposome conjugates, CpG, and anti-IL-10 mAbs were completely eradicated. In those mice, the frequency of CD8+ T cells reactive with OVA257–264 peptides in the context of H-2Kb was significantly increased. These results suggested that, by choosing lipid components for liposomes, surface-coupled liposomal Ags might be applicable for the development of tumor vaccines to present tumor Ags to APCs and induce antitumor responses.