Introduction. Worldwide, in children of under five years of age, diarrhea is responsible for more than 760,000 annual deaths. It is treated with both modern drugs and traditional medicinal plants, including O. lamiifolium. But the use of this plant as an antidiarrheal agent is not scientifically validated. Therefore, this study was aimed to evaluate antidiarrheal efficacy of the plant. Method. The leaf powder was macerated by 80% methanol and then fractionated using n-hexane, n-butanol, and distilled water. Antidiarrheal activity was evaluated through different models (castor oil-induced diarrhea, enteropooling, and motility) using onset, number of wet feces, fluid content, weight and volume of intestinal content, and motility as test parameters by administering treatment doses to groups of mice. Group I received 10 mL/kg of the dissolving vehicle, Group II received either loperamide or atropine, and Groups III-V received extract doses of 100, 200, and 400 mg/kg, respectively. One-way ANOVA was used to analyze the data, followed by Tukey’s post-hoc test. Results. The crude extract exhibited a significant effect on the fluid content of feces at all tested doses. Additionally, the n-butanol and distilled water fractions revealed significant effects on onset of diarrhea at 400 mg/kg ( p < 0.05 ), while the n-hexane fraction showed significant effects on number of wet feces, onset, and fluid content of feces at all tried doses. The crude extract and all the fractions (at 200 and 400 mg/kg) decreased the weight and volume of intestinal content significantly. Similarly, both the crude extract and distilled water fraction at 400 mg/kg as well as n-butanol and n-hexane fractions at 200 and 400 mg/kg showed meaningful differences on peristaltic index as compared to the negative control. Conclusion. The results revealed that the leaf extract of O. lamiifolium has an antidiarrheal activity, which supports the traditional medical practice.

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Background. Malarial infection has significant negative impact on the health of the world population. It is treated by modern and traditional medicines. Among traditional medicinal plants, Acacia tortilis is used by different communities as antimalarial agent. Therefore, the objective of this study is to validate antimalarial activity of the stem bark of Acacia tortilis in mice. Methods. To evaluate antimalarial activity of the plant, 4-day suppressive, curative, and prophylactic antimalarial test models were used. Parasitemia, packed cell volume (PCV), survival time, rectal temperature, and body weight were used to evaluate the effect of the plant extracts. Data were analyzed using SPSS version 26 followed by Tukey’s post hoc multiple comparison test. Results. The crude extract and dichloromethane fraction significantly suppressed the level of parasitemia ( p < 0.001 ) and increased mean survival time ( p < 0.01 ) at all tested doses. Similarly, significant effects were observed in mean survival time, % change of PCV, weight, and temperature in both curative and prophylactic antimalarial test models. Conclusions. The methanolic extract and solvent fractions of the stem bark of Acacia tortilis has shown antimalarial activity, and the finding supports the traditional use and the in vitro studies. Thus, this study can be used as an initiation for researchers to find the most active phytochemical entity and to conduct additional safety and efficacy tests.