ResumoEste estudo determinará a patogenicidade de bactérias isoladas das águas pantanais e fezes de corvos den-tro do campus UW (University of Washington) Bothell. Este estudo emanou da necessidade de determinar se o corvo americano (Corvus brachyrhyncos) tem um papel na epidemiologia da doença diarréica, junta-mente com sua contribuição significativa para o alto nível de coliformes fecais na água do fluxo que per-corre a área onde as aves se alojam. Modificado a partir de estudos anteriores, desenvolvemos protocolos para cultura de Escherichia coli e Campylobacter, ambas conhecidas por serem patogênicas e presentes em fezes de corvos, isolar DNA de culturas ou amostras e realizar PCR/qPCR para detectar genes de virulên-cia. Descobriu-se que os genes de virulência eae e rfb que são necessários para causar diarreia estavam au-sentes num número representativo de estirpes de E. coli isoladas das amostras de água e das amostras fecais. Os genes de virulência flaA e cad em espécies de Campylobacter foram detectados em amostras fecais (77,8% e 73%, respectivamente) e em amostras de água (75% cada). Concluindo, nossa hipotese não pôde ser confirmada, mas nossos resultados sugerem que a Campylobacter isolada das amostras de água e de fezes de corvo é potencialmente patogênica. No entanto, os resultados não são conclusivos e mais amostras e genes de virulência específicos para E. coli extraintestinal patogênica necessitam ser identificados, a fim de avaliar com precisão a patogenicidade destas bactérias. Palavras-chave: AbstractThis study will determine the pathogenicity of bacteria isolated from the wetland waters and crows feces within the UW Bothell campus. This emanated from the need to determine whether the American crow (Corvus brachyrhyncos) has a role in the epidemiology of diarrheal disease along with its significant con-tribution to the high level of fecal coliforms in the stream water that runs through the crow roosting area. Modified from previous studies, we developed protocols to culture Escherichia coli and Campylobacter, both are known to be pathogenic and present in crow feces, to isolate DNA from cultures or samples, and to perform PCR (Polymerase Chain Reaction)/qPCR (Quantitative Polymerase Chain Reaction) to detect virulence genes. We found that the virulence genes eae and rfb that are necessary to cause diarrhea were absent in a representative number of E. coli strains isolated from the water samples and the fecal samples. The virulence genes flaA and cad in the Campylobacter species were detected in fecal samples (77.8% and 73%, respectively) and in water samples (75% each). In conclusion, our hypothesis could not be verified, but our results suggest that the Campylobacter isolated from wetland water and crow feces are potentially pathogenic. However, the results are not conclusive and more sample and virulence genes specific to ex-traintestinal pathogenic E. coli, need to be screened in order to accurately assess the pathogenicity of these bacteria.
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