Root-knot nematode management requires the adoption of integrated practices. Biological agents and cultural control practices are the most widely used, but little is known about their combined effects. This study aimed to assess the interaction effects of the biological agents Trichoderma harzianum + Purpureocillium lilacinum and different green manures on the control of Meloidogyne javanica in soybean under greenhouse conditions. Green manures from white oat, Urochloa ruziziensis, Crotalaria spectabilis, millet, and buckwheat were grown separately and applied onto the soil surface. Subsequently, soybean seeds were treated with the biological agent and planted. The experiment was repeated twice to confirm the results. In Trial 1 and 2, application of green manure or biological treatment alone was efficient in reducing nematode populations. In Trial 1, there was an interaction between factors on total nematode number and number of nematodes per gram of root. Combined use of biological control with white oat and millet green manure produced great results, since when associated with the biological one, the reduction in the total number of nematodes was potentiated by 55 and 49%, respectively (Trial 1). There was no interaction between green manure and biological factors for Trial 2, and the best results were observed with green manures of C. spectabilis, U. ruziziensis and white oat, with a reduction in the population density of the nematode in 60, 59 and 44%, respectively. It is concluded that green manure application and T. harzianum + P. lilacinum were effective in reducing nematode populations when applied separately. White oat and millet green manures associated with T. harzianum + P. lilacinum increase thecontrol of M. javanica in soybean.