The episodes of diarrhea caused by neonatal bovine rotavirus group A (BoRVA) constitute one of the major health problems in the calf rearing worldwide. The main G (VP7) and P (VP4) genotypes of BoRVA strains involved in the etiology of diarrhea in calves are G6P[1], G10P[11], G6P[5], and G8P[1]. However, less frequently, other G and P genotypes have been described in BoRVA strains identified in diarrheic fecal samples of calves. This study describes the identification and molecular characterization of an emerging genotype (G6P[11]) in BoRVA strains involved in the etiology of a diarrhea outbreak in beef calves in a cattle herd of high production in extensive management system. The diarrhea outbreak, which showed high morbidity (60%) and lethality (7%) rates, occurred in calves (n= 384) Nelore (Bos indicus) up to 30-day-old from the State of Mato Grosso do Sul, Brazil. BoRVA was identified in 80% (16/20) of the fecal samples analyzed by polyacrylamide gel electrophoresis (PAGE) technique. In all PAGE-positive fecal samples were amplified products with 1,062-bp and 876-bp in the RT-PCR assays for VP7 (G type) and VP4 (VP8*) (P type) of BoRVA, respectively. The nucleotide sequence analysis of VP7 and VP4 genes of four wild-type BoRVA strains showed G6-III P[11]-III genotype/lineage. The G6P[11] genotype has been described in RVA strains of human and animal hosts, however, in calves this genotype was only identified in some cross-sectional studies and not as a single cause of diarrhea outbreaks in calves with high morbidity and lethality rates as described in this study. The monitoring of the G and P genotypes of BoRVA strains involved in diarrhea outbreaks in calves is important for both animal and public health by allowing the identification of the most frequent genotypes, the characterization of novel genotypes and to identify reassortments with genotypes described in animal and human hosts. The results of this study show the importance of the monitoring of the genotypes of BoRVA strains involved in episodes of bovine neonatal diarrhea as for characterization of frequency of occurrence and pathogenic potential of uncommon genotypes as for monitoring of the emergency of different BoRVA genotypes not included in commercial vaccines.

Calf diarrhea causes substantial economic losses in the cattle industry worldwide. Bovine rotavirus A (RVA) is the main viral agent that leads to enteric infection and diarrhea outbreaks in calves throughout the world. The aim of this retrospective (2006-2015) study was to determine the frequency of RVA detection in diarrheic fecal samples from beef and dairy calves from the three main cattle-producing regions of Brazil. Diarrheic fecal samples (n=1,498) of 124 beef and 56 dairy cattle herds from the Midwest, South, and Southeast geographical regions of Brazil were evaluated using the silver-stained polyacrylamide gel electrophoresis (ss-PAGE) technique. RVA double stranded-RNA was identified by the ss-PAGE technique in 410 (27.4%) fecal samples. The frequency of positive samples found in beef calves (31.9%; 328/1,027) was higher than the frequency found in diarrheic fecal samples from dairy calves (17.4%; 82/471). RVA infection was identified in calves from the three Brazilian geographical regions analyzed. However, the frequency of positive diarrheic calves in the Midwest region (39.4%), predominantly beef calves, was higher than in the South (19.4%) and Southeast (17.6%) regions. The temporal distribution of RVA-infected calves evaluated by two five-year periods (2006-2010, 24.5%; 2011-2015, 28.8%) demonstrated a very similar frequency of RVA in both periods. Considering the wide regional and temporal scope of this study, it can be concluded that RVA remains an important etiology of neonatal diarrhea in calves of Brazilian cattle herds.

No abstract

Resumo: Pesquisa exploratório-descritiva acerca da Hotelaria para cães e gatos na cidade de São Paulo, a fim de demonstrar as características e tendências e contribuir para os estudos aplicados sobre esse segmento turístico. Inicialmente enfoca o chamado "mercado pet", apresentando um breve contexto com dados gerais no mundo, e a evolução da hotelaria para animais no Brasil. Em seguida, trata da hospedagem para animais de estimação, a partir de dados sobre diferentes meios de hospedagem e suas características peculiares, e da visão de gerentes de hotéis que aceitam animais de estimação junto com os hóspedes e de proprietários de animais de estimação na zona sul dessa cidade. Os resultados obtidos indicam um segmento de mercado em ascensão em dois sub-segmentos: meios de hospedagem para animais e hotéis que aceitam animais junto com seus hóspedes. A oferta de produtos e serviços hoteleiros indica a incorporação do conceito de hospitalidade, a diversificação da oferta, a necessidade de profissionais capacitados e o desenvolvimento de sistemas administrativos e gerenciais específicos, considerando, hábitos, necessidades e expectativas dos donos de animais, ao lado das características de cada tipo de animal em particular. Palavras chave: Turismo e Hotelaria. Meios de hospedagem. Mercado pet. Características e tendências. São Paulo (cidade)/Brasil.Abstract: This paper focuses on Hotels for dogs and cats in São Paulo city. Characteristics and tendencies are analyzed aiming to contribute to applied studies on the subject. It begins by bringing general data on pet market in the world and on hotels for pets in Brazil. Pets hosting is assessed through registers on the subject and interviews with managers of that kind of hotels, as well as with pet owners living in the southern part of São Paulo city. It could be stated that pet hosting is a rising 1 Bacharel em Turismo pela Universidade Anhembi Morumbi.