The high brachial artery bifurcation is an anatomical variation found in the arm segment. Although it does not present any alteration in the arterial blood functionality, it is considered a common spot for vascular lesions during surgical interventions that consist of the absence of anatomical knowledge. The research objective was to describe the high brachial artery bifurcation found in a cadaveric specimen from the anatomy laboratory as well as its main anatomoclinical aspects. Twenty-six upper limbs were investigated and dissected from the Universidade Brasil's Human Anatomy Laboratory, SP. These were formolized cadaveric specimens from both sexes. During upper limb dissection, arterial anatomical variations were observed in a single-arm segment. The variation was unilateral in the left hemisphere. The clinical findings were high brachial artery bifurcation and a rare case of the radial artery in the medial path, as well as an ulnar artery with a lateral path in the arm median third. It is clear that the understanding of anatomy and anatomical variations patterns is of utmost importance and a requirement for surgery, so surgeons need to be aware of clinical, anatomical, and arterial variations data, avoiding vascular lesions during the surgical interventions. Dissection is an important learning tool for students and resident doctors. It is suggested the use of dissection as a pedagogical resource to acquire skills in surgeries during internship and also to improve the anatomical variation cognition of upper limbs.