Sodium diethyldithiocarbamate (DTC) forms stable colored [M II (DTC)2] complexes where M II = Mn, Fe, Co, Ni, Cu and Zn. The characterization data suggested square planar geometrical structure for Co II , Ni II and Cu II complexes and a tetrahedral structure for Mn II , Fe II and Zn II complexes. Six new complexes of general formula [M(DTC)2PAN] where PAN=1-(2-Pyridyl azo)-2-naphthol, were synthesized by using two different routes of preparation. The structures of the complexes are discussed in relation to the analytical, spectroscopic, magnetic and conductivity data. The DTC-ligand exhibits bidentate behavior acting as S,S`-donors in all complexes. PAN was also behave as bidentate N,N` ligand. The electronic spectra along with magnetic moment values suggested an octahedral structure for [M(DTC)2PAN] complexes. The biological activity for ligands and the prepared complexes were studied against Escherichia Coli, Pseudomonas (G -) and Staphylococcus Aureus, Bacillus (G + ) bacteria. Some of the complexes have a relatively strong deactivating capacity against the specimen of bacteria. The presence of PAN ligand increased the inhibition zone.