Chemical investigation of the lichen Dirinaria applanata led to isolate nine compounds including a new hopane derivative, 1-acetoxy-21-hopane-3,22-diol (1) together with six phenolic compounds naming divaricatinic acid (2), methyl divaricatinate (3), methyl--orcinolcarboxylate (4), methyl haematommate (5), divarinol (6), ramalinic acid A (7), and two xanthones namely lichenxanthone (8), 4,5-dichlorolichenxanthone (9). Their structures were elucidated by spectroscopic data in combination with published literature. Except compound 2, all compounds were isolated from this species for the first time.