In Vietnam, Anguilla eels have been documented before, but their species composition and biological characteristics are still relatively unknown. Research on the molecular phylogeny of Anguilla has been limited, and identification of the species of eels was usually based on morphological characteristics. This is the first time both cytochrome c oxydase subunit I (COI) and 16S ribosomal RNA (rRNA) genes were used to identify Anguilla eels in water bodies, where the eels can migrate downstream and upstream during different stages of the life cycle, in Thua Thien Hue province, Vietnam. Based on DNA sequence analysis of six specimens, two Anguilla species (including subspecies) were confirmed as A. marmorata and A. bicolor pacifica, which had not been identified based on morphological characteristics in previous analyses. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (4): gmr18722 K.T. Huyen et al. 2 Phylogenetic analysis showed that the Anguilla genus in Thua Thien Hue province belongs to the Indo-Pacific lineage and helped build phylogenetic trees generated from mtDNA data from GenBank for these eels.

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Breast cancer (BC) is a complex disease throughout the world and it is one of the most common cancer among women both in developed and developing countries. To enhance the survival of BC patients, genetic factors are used for early diagnosis because they are non-changed factors and present ability of cells to proliferate and metastasize. MiR-608 targets many genes which are vital for development, differentiation, motility, apoptosis and angiogenesis. The SNP rs4919510 (C/G) affects the processing of the pre-miRNA to its mature form and the ability to regulate target genes. This SNP has been demonstrated to relate to breast cancer in Chinese and Iranian population. Our study aimed to investigate the association between the SNP rs4919510 and BC in Vietnamese population. 106 cases and 101 controls were genotyped using optimized tetra-ARMs-PCR method, then statistical analysis was applied to examine the correlation of the SNP. The results show that this SNP is high polymorphism with the frequencies of minor allele C is 24.8% and major allele G is 75.2% in Vietnamese population. Statistic result revealed an obvious increased risk of BC among Vietnamese women when compared of heterozygote model and dominant model (CG vs. GG: OR=1.93, 95% CI: 1.09-3.45, p= 0.02; CC+CG vs. GG: OR=1.83, 95%CI: 1.05-3.17, p=0.03). Our study suggested that the polymorphism of rs4919510 may be associated with BC risk in Vietnamese individuals.

Petrographical characteristics and post-depositional alteration studies of sandstones are the two important factors to reservoir rocks, which affect oil and gas storage and permeability of reservoir rocks. This study revealed petrographical characteristics, post-depositional alteration, and their influence on the porosity and permeability of Oligocene sandstones, including C, D, and E and F sequences, block 15-1/05, Cuu Long Basin. The results show that most of the sandstones were arkose, lithic arkose, and sporadically interbedded by feldspathic greywacke. The post-depositional alteration was progressively increasing following the burial depth from early diagenesis of sequence C, to intermediate diagenesis of sequence D and advanced diagenesis of sequence E and F. The post-depositional alterations significantly influenced on the porosity of the Oligocene sandstone were the cementation and mechanical compaction. They reduced the porosity and permeability of the sandstone. Additionally, authigenic clay minerals have a negative effect on permeability in which sandstones were rich illite and illite-smectite clay minerals, and the permeability tended to decrease stronger than others. Our results showed that the potential reservoir rocks of Oligocene sandstones, block 15-1/05 were sequence E and F sandstones that are in well grain sorting, well grain roundness shape, and contained a small number of cement, particularly the absence of illite and illite-smectite.