Introduction. Antimicrobial resistance (AMR) is a One Health issue concerning humans, animals and the environment and a unified One Health approach is required to contain this problematic issue. Dogs and cats are popular pet animals and are known to carry many bacterial pathogens that are of public health importance, including Salmonella . However, data on AMR in companion animals is limited. Gap statement. Scant AMR data from bacteria originating from companion animals limits an accurate assessment of the impacts of pet-animal-related AMR on public health. Purpose. This study aimed to phenotypically and genetically investigate AMR in Salmonella isolated from pet dogs and cats in Thailand. Methodology. Salmonella enterica were isolated from pet dogs (n=159) and cats (n=19) in Thailand between 2016 and 2019. All isolates were serotyped. Phenotypic and genotypic antimicrobial resistance was examined. PCR-based replicon typing, replicon sequence typing and plasmid multilocus sequence typing were conducted to characterize plasmids. Results. Seventy-seven serovars were identified, with serovars Weltevreden (9.6%) and Stockholm (9.0%) the most common. Most of the isolates (34.3%) were multidrug-resistant. The serovar Stockholm was an ESBL-producer and carried the β-lactamase genes bla TEM-1 and bla CTX-M-55. The plasmid-mediated quinolone resistance (PMQR) gene, qnrS, was also detected (10.1%). Class 1 integrons carrying the dfrA12-aadA2 cassette array were most frequent (45.9%). Five plasmid replicon types as IncA/C (0.6%), N (1.1%), IncFIIA (28.7%), IncHI1 (2.2%), and IncI1 (3.4%) were identified. Based on the pMLST typing scheme (n=9), plasmids were assigned into five different STs including IncA/C-ST6 (n=1), IncH1-ST16 (n=4), IncI1-ST3 (n=1), IncI1-ST60 (n=1) and IncI1-ST136 (n=1). The ST 16 of IncHI1 plasmid was a novel plasmid ST. Subtyping F-type plasmids using the RST scheme (n=9) revealed four different combinations of replicons including S1:A-:B- (n=4), S1:A-:B22 (n=2), S3:A-:B- (n=1) and S-:A-:B47 (n=1). Conclusions. Our findings highlight the role of clinically healthy household dogs and cats as carriers of AMR Salmonella strains with different R plasmid. The implementation of AMR phenotypes instigation and genotypic monitoring and surveillance programmes in companion animals are imperative as integral components of the One Health framework.
Antimicrobial resistance (AMR) is One Health issue involved in humans, animals and environment and a unified One Health approach is required to contain this problematic issue. However, data on AMR in companion animals is limited. Dogs and cats are popular pet animals and are known to carry many bacterial pathogens that are of public health importance, including Salmonella. This study aimed to identify serovars of Salmonella and phenotypically and genetically characterize AMR in Salmonella isolated from dogs and cats in Northern and Northeastern Thailand. A total of 178 S. enterica isolates from dogs (n= 159) and cats (n= 19) collected between 2015 – 2018 were included. Seventy-seven serovars were identified, of which serovars Weltevreden (9.6%), Stockholm (9.0%), and Typhimurium (7.3%) were most common. The majority of the isolates (34.3%) were multidrug resistant. Only one ESBL-producing isolate (i.e., serovar Stockholm) was identified and the isolate carried blaTEM-1 and blaCTX-M55. Of all plasmid-mediated quinolone resistance genes tested, only qnrS (10.1%) was detected. Plasmid replicon types found were IncA/C (0.6%), N (1.1%) IncFIIA (28.7%), IncHI1 (2.2%), and IncI1 (3.4%). IncFIIA was most prevalent (51 isolates, 28.7%). Based on the pMLST typing scheme, the plasmids were assigned to 5 different STs including IncA/C-ST6, IncH1-ST16, IncI1-ST60, IncI1-ST101, and IncI1-ST136, of which ST 16, of IncHI1 plasmid was the novel plasmid STs. Subtyping F-type plasmids using the RST scheme revealed 4 different combinations of replicons including S1:A-:B- (n=4), S1:A-:B22 (n=2), S3:A-:B- (n=1), and S-:A-:B47 (n=1). The findings highlight the role of household dogs and cats as carriers of AMR Salmonella enterica strains with R plasmid and AMR determinants. These Salmonella strains may spread to humans and environment and promote the wide distribution of AMR.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.