The hierarchical zeolite is one of the most promising materials for catalytic applications. However, the effect of its pore connectivity on catalytic behaviors and coke formation has not clearly been revealed. In this contribution, we demonstrate the visualization of the mesopore architecture in three-dimensional perspectives together with the pore connectivity network of poreopened hierarchical mordenite (MOR), fabricated by the seedassisted template-free synthesis followed by the fluoride treatment via the electron tomography (ET) technique. Interestingly, the pore-opened zeolites clearly display higher catalytic performance (approximately 80% of ethylene yield) in ethanol dehydration with respect to the parent one due to their additional pore-opened structures connected to the external surfaces of zeolites. In addition, the effect of pore connectivity network on the coke location and type obtained from ethanol conversion has been observed. It was found that the porous structure of the etched sample is directly connected to the external surface, and then, the large area of crystals can contribute to the reaction. Conversely, only a small amount of closed mesopores is observed inside the crystals in the case of the untreated sample, and therefore, the molecules cannot easily penetrate inside crystals for the catalytic reaction. These results open up promising perspectives for the development of hierarchical catalysts including fabrication by the template-free synthesis approach, pore-architecture characterization, and catalytic applications.

Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30°C. Electron microscopy; phylogeny based on nine conserved taxonomically informative markers, on ompA, or on seven housekeeping genes; and whole-genome sequencing and analysis of the isolate confirmed the identity of the isolate as a new member of the genus Chlamydia, a new species that we name Chlamydia crocodili.

Pseudomonas aeruginosa is a notable nosocomial pathogen that can cause severe infections in humans and animals. The emergence of multidrug resistant (MDR) P. aeruginosa has motivated the development of phages to treat the infections. In this study, a novel Pseudomonas phage, vB_PaeS_VL1 (VL1), was isolated from urban sewage. Phylogenetic analyses revealed that VL1 is a novel species in the genus Litunavirus of subfamily Migulavirinae. The VL1 is a virulent phage as no genes encoding lysogeny, toxins or antibiotic resistance were identified. The therapeutic potential of phage VL1 was investigated and revealed that approximately 56% (34/60 strains) of MDR P. aeruginosa strains, isolated from companion animal diseases, could be lysed by VL1. In contrast, VL1 did not lyse other Gram-negative and Gram-positive bacteria suggesting its specificity of infection. Phage VL1 demonstrated high efficiency to reduce bacterial load (~ 6 log cell number reduction) and ~ 75% reduction of biofilm in pre-formed biofilms of MDR P. aeruginosa. The result of two of the three MDR P. aeruginosa infected Galleria mellonella larvae showed that VL1 could significantly increase the survival rate of infected larvae. Taken together, phage VL1 has genetic and biological properties that make it a potential candidate for phage therapy against P. aeruginosa infections.

Bifunctional acid‐base catalysts have been developed by fabricating core‐shell faujasite@aqueous miscible organic‐layered double hydroxides (FAU@AMO‐LDHs) composites via the growth of AMO‐LDHs on zeolite surfaces. Interestingly, by adjusting the acid/base properties of the composites, the one‐pot synthesis of ethyl trans‐α‐cyanocinnamate via deacetalization‐Knoevenagel reaction can be achieved up to 90% yield. In contrast, almost no desired products can be observed in cases of isolated zeolites and AMO‐LDHs, demonstrating the synergistic effect of zeolites and AMO‐LDHs as acid‐base catalysts. These findings open up new perspectives of the development of tunable acid‐base zeolite@LDHs composites in an acid‐base tandem reaction, which can selectively produce ethyl trans‐α‐cyanocinnamate up to 90% yield.