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High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection.

This study indicated that nacre promotes ALP, BSP, and OC gene expression.

Objectives:The study aimed to compare the residual forceoflocally produced Thai orthodontic elastomeric ligatureswith 2 commercial brands.Materials and Methods: The study compared Thai clear and blue orthodontic elastomeric ligatures with clear and blue commercial brands[Unitek (USA) and W&H (China)]. The dimensional characteristics of the ligatures (i.e., inner diameter, outer diameter and cross-section thickness) were measured. Then initial extension force was measured. After that, the residual force was determined at day 1and repeated on day 2,3,4,5,6,7,14,21, and 28 andthe percentage of residual force wascalculatedaccording to ISO 21606:2007. The data were analyzed with Two-way ANOVA and multiple linear regression (p<0.05). Results: Thai ligatures had significantly greater inner diameter(clear 1.22 mm, blue 1.21 mm) and cross-section thickness (clear 0.78 mm, blue 0.79 mm) in both colors when compared with Unitek and W&H brands. Thai ligatures had no significant difference in cross-section thickness between clear and blue.Thai clear ligatures hadthe highest initial extension force(2.30 N). Thai ligatures showed similar force decay patterns as other brands, rapidly decreasing the first day, gradually decreasing over 7 days and remaining nearly constant until 28 days.However, both clear and blue Thai elastomeric ligatures had significantly less outer diameters (clear 3.12 mm, blue 3.15mm). Thai blue ligatures had the lowest initial extension force (1.96 N).TheThai clear and blue demonstrated less percentage of residual force in the first day (clear 53.00%, blue 50.66%) and28 days (clear, 37.69%, blue, 37.00%) butthey were clinically acceptable. In general, clear ligatures exhibited a greater percentage of residual force than blue ligatureamong the 3 brands. Conclusion:Thai orthodontic elastomeric ligature properties are comparable to commercially available brands and acceptable for clinical application.