We have previously identified a candidate oncogene (PRAD1 or D11S287E) on chromosome 11q13 which is clonally rearranged with the parathyroid hormone locus in a subset of benign parathyroid tumours. We now report that a cloned human placental PRAD1 complementary DNA encodes a protein of 295 amino acids with sequence similarities to the cyclins. Cyclins can form a complex with and activate p34cdc2 protein kinase, thereby regulating progress through the cell cycle. PRAD 1 messenger RNA levels vary dramatically across the cell cycle in HeLa cells. Addition of the PRAD1 protein to interphase clam embryo lysates containing inactive p34cdc2 kinase and lacking endogenous cyclins allows it to be isolated using beads bearing p13suc1, a yeast protein that binds cdc2 and related kinases with high affinity and coprecipitates kinase-associated proteins. Addition of PRAD1 also induces phosphorylation of histone H1, a preferred substrate of cdc2. These data suggest that PRAD1 encodes a novel cyclin whose overexpression may play an important part in the development of various tumours with abnormalities in 11q13.

that servers hosted was documented, and on July 6, it was documented whether servers allowed health sciences discipline selection during the submission process and whether they hosted more than 500 such preprints (servers' health sciences categories are listed in the data repository site).Results | We analyzed 57 preprint servers that hosted approximately 3 million preprints in total. Of those, 10 servers hosted more than 500 health sciences preprints (Table 1). Of the 7 analyzed policies, the most commonly addressed across all servers was screening of preprints before or after they are made public (n = 47 [82%]) (Table 2). Two servers, Preprints.org and Research Square, used a screening checklist (the latter also provided a "badge" of passed checks). The most commonly addressed submission requirements were specifying the scholarly scope of preprints (n = 57 [100%]) and the study type allowed for deposit (n = 31 [54%]). Of the 18 analyzed recommendations on transparency in reporting and research integrity, preprint servers addressed a median of 1 recommendation (range, 0-11), most commonly data sharing (n = 22 [39%]). These recommendations were more prevalent (median, 5; range, 0-11) for the 10 servers with more than 500 health sciences preprints.

Announcing the launch of medRxiv for faster access to better evidence

The new PLOS Data Policy will require all submitting authors to include a data availability statement as of March 1, 2014. Read more about what this means and see the policy itself in this cross-PLOS Editorial from Theo Bloom and colleagues. Please see later in the article for the Editors' Summary