Objective The purpose of the present study was to investigate healthcare workers’ physical exercise levels linked to their quality of life. Healthcare workers’ from all departments of a General hospital participated in the study. The instruments used for data collection regarding quality of life and physical exercise (Short Form Health Survey (SF-36) and International Physical Activity Questionnaire-short form). Results Regarding the lack of physical exercise, the participants mainly put the blame on lack of free time (58%, n = 106), work hours (41% n = 75), but also pure negligence (37%, n = 67). The SF-36 scores showed that the existence of health problems can affect in a negative way and aggravate almost every quality of life parameter. Regarding physical activities in the past 7 days prior to the survey, most of them were about housekeeping and household-related chores (42.3%), followed by out-of-the-house errands (13.2%). There were also differences among mental health and postgraduate education level. According to our findings, a major factor that could boost healthcare professionals’ physical activity, is to increase knowledge and raise awareness about the benefits linked to physical activity.