Background: Today, conservation and development of indigenous knowledge of medicinal plants are the urgent demand of science. Uvaria grandiflora Roxb. ex Hornem which is used by medicinal experience of people of ethnic minorities in Quang Tri Province has shown in vitro cytotoxic activity in our previous screening tests. This article is an ongoing research to determine the chemical ingredients of this species. Materials: The aerial parts of Uvaria grandiflora Roxb. ex Hornem were collected from Quang Tri Province. Method: Fractional extraction was performed by using organic solvents. Volatile components were analysed by gas chromatography – mass spectrometry (GC-MS and indentified by comparison with published mass spectra database Wiley and NIST. Results: Two major components are δ-cadinene (42.94%) and caryophyllene (9.51%). Some ingredients that consist of γ-muurolene, ar-curcumene, calarene, β-vetivenene, β-bisabolene, cadina-1,4-diene and calacorene have never been reported from U.grandiflora collected from Quang Tri province. According to others references, some of these compounds have abundant bioactivities such as antimicrobial, antioxidant, antiinflammatory activities, especially α-humulene and caryophyllene with anticancer activities in vitro. Fractional extraction was performed by using organic solvents. Volatile components were analysed by gas chromatography – mass spectrometry (GC-MS and indentified by comparison with published mass spectra database Wiley and NIST. Conclusion: A total of sixteen volatile compounds were indentified in n-hexane fraction of Uvaria grandiflora Roxb. ex Hornem. Key words: Uvaria grandiflora, δ-cadinene, α-humulene, caryophyllene