In this study, we determined the phytocomponents using GC-MS and evaluated antioxidant activity by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay of the methanolic extract of six lotus varieties seed in Viet Nam (N. Nucifera). GC-MS chromatogram of the methanolic extract of six lotus varieties seed in Viet Nam showed 27 peaks which indicate the presence of 27 phytochemical constituents. The major phytochemical constituent is methyl-alpha-dgalactopyranoside (66,86-78,69%) in all lotus varieties. Antioxidant activity of liquid extract and condensed extract was found as 8.20-15.67 mg/mL and 0.850-1.286 mg/mL in comparison with IC50 of ascorbic acid (3.2 μg/mL). Moreover, 50 % of the DPPH scavenging ability of liquid and condensed extract differed from each other. From obtained results, the seeds of six lotus varieties in Viet Nam were found to be potential for bioactive compounds and antioxidant activity.